Snmek 1

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E N Z Y M A T I C D E C O N T A M I N A T I O
N O F S U L F U R M U S T A R D
Masaryk University Enantis, s.r.o. VOP-026
ternberk, s.p., division VTÚO Brno CPN, s.r.o.
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W a r f a r e a g e n t s u l f u r m u s t
a r d

• used for the first time in 1917 near Belgium city
  Ypres
• easily penetrates to various materials
• makes burn marks and blisters due to skin
  contacts
• military use banned by international convention
• easy to prepare from common chemicals

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T e c h n o l o g y d e v e l o p m e n t

• Czech Republic development of enzymatic
  decontamination mixturefor sulfur mustard
  prioritized by NATO
• research projects funded by Ministry of Defence
  2004 2006 and Ministry of Education 2005
  2011
• degradation of sulfur mustard by enzymes
  dehalogenases demonstrated
• protection of intellectual properties Czech
  patent CZ 298287
• technology transfer to practice biotechnology
  spin-off Enantis

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H a l o a l k a n e d e h a l o g e n a s e s
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H a l o a l k a n e d e h a l o g e n a s e s
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T o x i c s u l f u r m u s t a r d
S
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N o n t o x i c a n a l o g u e
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C o m p u t e r m o d e l l i n g
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C o m p u t e r m o d e l l i n g
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C o m p u t e r m o d e l l i n g
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C o m p u t e r m o d e l l i n g
sulfur mustard
bis(2-chloroethyl) ether
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E x p e r i m e n t a l t e s t s
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E x p e r i m e n t a l t e s t s
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P a t e n t - p r o t e c t e d c o n c e p t
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R e p o r t s i n s c i e n t i f i c j o u r
n a l s
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T e c h n o l o g y t r a n s f e r t o p r a
c t i s e

• development of decontamination mixture
• optimisation of protein production
• large-scale industrial production
• product commercialization
• development of new and improved products

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P r o d u c t d e v e l o p m e n t a n d t
e s t i n g

• testing of dehalogenases in decontamination
  aerosols(Pacific Northwest National Laboratory
  USA)
• testing of dehalogenases in polymer
  particles(Foster Miller, US Army USA)
• testing of dehalogenases in microemulsions(Defenc
  e Science and Technology Laboratory Great
  Britain)
• testing of dehalogenases in skin decontamination
  cream(Bundeswehr Institute of toxicology and
  pharmacy Germany)
• NATO testing of enzyme decontamination on
  aircraft interriers

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C o n s o r t i u m o f p a r t n e r s

• Masaryk University and Enantis, s.r.o.
• 14 years of research on dehalogenases
• gt 40 genes, gt100 protein variants
• know-how from 29 research stages
• modern laboratories and facilities for protein
  engineering
• E N Z Y M E D I S C O V E R Y A N D O P T I M
  I Z A T I O N
• isolation and construction of new enzymes
• genetic breeding of enzymes with improved
  catalytic properties

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C o n s o r t i u m o f p a r t n e r s

• Masaryk University and Enantis, s.r.o.
• VOP-026 ternberk, s.p., division VTÚO Brno
• applied research and development of
  decontaminations and defence against warfare of
  mass destruction
• personal and infrastruture based on 50 years of
  experience
• T E S T I N G O F E N Z Y M E S W I T H W A R
  F A R E A G E N T S
• study of warfare chemicals with extreme toxic
  properties
• compatibility testing of individual components
  for decontamination mixtures

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C o n s o r t i u m o f p a r t n e r s

• Masaryk University and Enantis, s.r.o.
• VOP-026 ternberk, s.p., division VTÚO Brno
• CPN, s.r.o.
• Production of pharmaceuticals and cosmetics
• large-scale industrial fermentations and
  down-stream processing
• P R O C E S S S C A L E U P A N D P R O D U
  C T I O N
• optimization of enzyme production, pilot-scale
  and full production
• development of protective cream for skin
  decontamination

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E x p e r i e n c e s w i t h t e c h no l o g
y t r a n s f e r

• high-quality basic research
• systematic and selective financial support
• investment into human resources
• competitive infrastructure
• financial support and consulting for protection
  of intellectual properties
• business consulting and administrative support
• collaboration with local industrial partners

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E x p e r i e n c e s w i t h t e c h no l o g
y t r a n s f e r

• high-quality basic research
• systematic and selective financial support
• investment into human resources
• competitive infrastructure
• financial support and consulting for protection
  of intellectual properties
• business consulting and administrative support
• collaboration with local industrial partners

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E x p e r i e n c e s w i t h t e c h no l o g
y t r a n s f e r

• high-quality basic research
• systematic and selective financial support
• investment into human resources
• competitive infrastructure
• financial support and consulting for protection
  of intellectual properties
• business consulting and administrative support
• collaboration with local industrial partners

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A c k n o w l e d g e m e n t s
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E x c u r s i o n t o l a b o r a t o r i e s
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E x c u r s i o n t o l a b o r a t o r i e s

• established 1994, funded by industry, 1
  laboratory
• until today 18 grants, 6 international
• laboratories 5, instrumentation 1 mil EUR
• in parallel 12 research projects
• know-how from 29 research stages, 11 visitors
• courses Bioinformatics and Biocatalysis

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E x c u r s i o n t o l a b o r a t o r i e s
3 doctors, 10 doctoral students, 5 diploma
students, 1 technician