EDS

1
EDS

• Egg Drop Syndrome
• Recombinant Vaccine

2
Introduction

• EDS is an Infectious viral disease of laying
  hens.
• EDS cause reduction in egg production and
  production of Thin-Shell or Shell-less eggs.
• First observed in chickens in 1976 in Holland
• The disease cause a considerable Economic losses.

3
EDS virus structural characteristics

• Adenoviruses are Double-stranded DNA
  non-enveloped icosahedral particles.
• A penton fiber projects from each apex.

4
Identify a subunit to be used as a vaccine
5
Efficiency of recombinant protein in HI and SN
tests
E.Fingerut at el Vaccine 21 (2003) 2761-2766
6
Advantages of subunit vaccines

• A well defined, stable, clean antigens.
• The immune response is highly specific, it is
  producing protective Ab only and not against
  irrelevant antigens. (guided missile attack ver.
  carpet bombing)
• The immune response can be precisely examined.
• Are easily produced by recombinant DNA technology.

7
Advantages of subunit vaccines

• The vaccine is highly accurate.
• The vaccine is produced by means of biotechnology
  (not in live poultry, eggs or tissue) and
  therefore it is free from animal tissue residues.
• High repeatability- every vaccine batch contain
  exactly the same ingredients as the previous and
  future batches.
• No problem from incomplete inactivation of the
  pathogen.