CLASSIFICATION:

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PUCCINIA

• CLASSIFICATION
• Kingdom Mycota
• Division Eumycota
• Subdivision Basidiomycotina

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• Class Teliomycetes
• Order Uredinales
• Family Pucciniaceae
• Genus Puccinia
• Species graminis

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• INTRODUCTION
• The genus includes about 3000 species of these
  147 has been reported from India.
• It causes a rust disease - black stem rust of
  Wheat.

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• Puccinia graminis is an obligate parasite.
• It is a heterocious parasite.
• Its life cycle on Wheat and on Barberry.
• Wheat is a primary host.
• Barberry is a secondary or alternate host.

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• Spore-producing stages (0, I, II, III, IV) in
  their life cycles.
• Puccinia graminis 0 and I on barberry bushes
  (Berberis vulgaris dicot)
• II and III on various grasses (monocot)
• IV on soil.

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Puccinia- Infected Plant Parts
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Stages on Wheat

• Acediospores geminates on Wheat stem or leaf to
  form binucleate, dikaryotic filamentous well
  developed, branched, intercellular mycelium.

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Dikaryophase Reproduction On Wheat Puccinia
reproduces by (Asexual) sporulation. The spores
are of two kinds(i) Uridiospores (2N)
Uridosporangium (ii) Telutospores
(2N) Telutosporangium(iii) Basidio spores
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• Each cell contains a pair of nuclei (n n)
  constituting a dikaryon.
• To obtain nutrition haplophase phase develop
  haustoria.

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• Uredineal Stage
• The hyphae of the dikaryotic mycelium - aggregate
  near the surface of the infected organ to form a
  hyphal mass known as Ureida.

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• The tip of each hyphae swells to form a single
  binucleate oval uredospore formed in a group.
  uredinium or uredosorus.
• They exert pressure on the epidermis, finally
  ruptured in form of slits through which rusty
  coloured Uredospores are seen.

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Puccinia- Rust diseases Uredosporangium
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Puccinia Uredo- sporangium
Uredospore
Mycelium
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Puccinia Uredo-sporangium-Wheat
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Uredospore are stalked, unicellular oval,
binucleate structures, rusty red in colour.
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• Telial Stage
• Late in growing season, Uredospores are
  metamorphoses into Telutospores in the same
  Uredosori.
• Uredosori is known as Telutosori.
• Telutospores are dark brown or black in colour.

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Stage III Telia bearing teliospores (nngt2n)
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Puccinia Teluto sporangium
Telutospores
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Telutospores Dark brown or black in colour.
stalked, two celled, spindle shaped.The wall is
thick, black and smooth.
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Puccinia teluto spores

• Each cell has two nuclei (one plus strain and the
  other of minus strain).
• final stage on primary host
• Telutospore matures, the nuclei in each cell fuse
  to form a diploid nucleus (Kayogamy) (2N).

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Germination of Teluto spore

• On Soil
• Favourable condition- Proper Temperature,
  Moisture
• Germination of Teluto spore- germination tube -
  Epibasidium

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Germination of Teliospore
Germination tube
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Germinated Teluto spore and formation of Basidium
Basidio spores
Basidio spore () Or (-)
Sterigmata
Epibasidium
Basidium
Hypobasidium
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Basidiospores( and -)
Basidiospores germinate on upper epidermis of
Barberry leaf and produce Pycniosporangium
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Stages of Puccinia on Barberry leaf

• Pycniospores stage On upper epidermis with ve
  and ve basidiospores (Sexual reproduction)
  (Plasmogamy)
• Aecidiospore stage On lower epidermis,
  Binucleate Aecidiospore

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Infection of Puccinia on Barberry leaf Lower and
Upper sides
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Puccinia Pycnium on Upper epidermis
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Receptive hyphae and Spermatia
Receptive hyphae
Spermatia
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Develop-ment of Aecidiosporangium
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Stage I Aecia bearingaeciospores (nn)
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Puccinia- Aecio sporangium on lower epidermis
aeciospores infect primary host.Aeciospores
produced on alternate host (e.g.,Barberry) infect
primary host (e.g., grasses)
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• Aeciospores- round, yellowish red, Unicellular,
  binucleate, thick walled, 6 germ pores.
• Germinate on wheat plant.

Aecidiosporangium
Aeciospores
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Puccinia- Polymorphic fungi

• Polymorphism Various types of spores produced
  during life cycle according to hosts life cycle
  and climatic condition.
• Known as Polymorphism.
• Fungi shows polymorphism known as Polymorphic
  fungi.

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Puccinia Life Cycle
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Puccinia life cycle
Puccinia Life Cycle
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