If the required RPM

1
Centrifugation
It is important that the tubes are equally
balanced in the centrifuge
If the required RPMs cannot be achieved by site
centrifuge, as calculated in FORMULA 1, then
perform the following 1) Calculate maximum
force (no. of g) that centrifuge is capable of,
using FORMULA 2. 2) Substitute this value into
FORMULA 3 to determinate the amount of time
required on the site centrifuge. Formula
1 RPM 1000 x square root (No. of g) / (11.18)
x radius (cm) Formula 2 g 11.18 (RPM/1000)2
x radius (cm) Formula 3 Time (site centrifuge)

This table refers to G 1500
RADIUS SPEED (cm) (rpm) RADIUS SPEED (cm) (rpm)
7 4 300 to 4 400 8 4 000 to 4 100 9 3 800 to 3 900 10 3 600 to 3 700 11 3 400 to 3 500 12 3 300 to 3 400 13 3 200 to 3 300 14 3 000 to 3 100 15 2 900 to 3 000 16 2 800 to 2 900 17 2 800 to 2 900 18 2 700 to 2 800 19 2 600 to 2 700 20 2 500 to 2 600 21 2 500 to 2 600 22 2 400 to 2 500 23 2 400 to 2 500 24 2 300 to 2 400 25 2 300 to 2 400 26 2 200 to 2 300
(Time required x No. of g required) No. of g of
site centrifugation
If you do not have a refrigerated
centrifuge If the facility does not
have access to a refrigerated centrifuge, the
centrifuge rotor and/or buckets/inserts
(dependent on the type of centrifuge) should be
chilled for several hours in the refrigerator
prior to centrifuging the blood sample. This
procedure will help to keep the sample(s) cool
during the centrifugation step and any condensate
that collects in the rotor buckets/inserts etc
between centrifuge use can be removed using
absorbent material.