tcr

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CellRapeutics TCR Technology

• Reference https//www.creative-biolabs.com/car-t/
  flow-cytometry-application-in-car-t-system.htm

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What is TCR?

• The T-cell receptor, or TCR, is a molecule found
  on the surface of T cells, or T lymphocytes,1
  that is responsible for recognizing fragments of
  antigen as peptides bound to major
  histocompatibility complex (MHC) molecules. The
  binding between TCR and antigen peptides is of
  relatively low affinity and is degenerate that
  is, many TCRs recognize the same antigen peptide
  and many antigen peptides are recognized by the
  same TCR.

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Introduction

• Altering the specificity of T cell receptor (TCR)
  is one of the popular strategies to genetically
  modify T cells to enhance the tumor-killing
  activity of T cells. From a tumor-reactive T cell
  or active anti-tumor T-cell antigens, the
  appropriate target sequence is introduced to
  modify T cells to target a broad range of tumors
  with improved specificity. With engineering TCR
  construction and a series of assay, such as
  transgene expression, TCR/CD3 stability,
  lymphocyte antigen reactivity and cytotoxicity,
  animal experiments, etc., we provide
  cGMP-compliant TCR products for preclinical and
  clinical trials.

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Structure and function of the TCR
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TCR Structure

• The T cell receptor (TCR), found on the surface
  of T cells, is responsible for antigen
  recognition. It consists of two chains the alpha
  (a) and beta (ß) chains. Both chains have a
  constant region (c) and a variable region (v),
  and it is the variable region that determines
  antigen specificity. The TCR is associated with
  the CD3 complex, which comprises three
  transmembrane signalling molecules (CD3??, CD3de
  and CD3?e).

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TCR Structure

• A TCR will interact with an antigen on a target
  cell when the target peptide sequence is
  presented by the appropriate major
  histocompatibility complex (MHC-1 for cytotoxic T
  cells). Efficient T-cell activation also requires
  the simultaneous binding of the T cell
  co-receptor (CD8 for cytotoxic T cells). ss,
  disulphide bridge.

7
Thanks

• Reference https//www.creative-biolabs.com/car-t/
  flow-cytometry-application-in-car-t-system.htm