A1258690256EplgY

1
(No Transcript)
2
DNA strand synthesis by formation of
phosphodiester bonds. The chain is terminated
by the use of dideoxycytidine triphosphate (ddC)
in place of deoxycytidine triphosphate (dCTP).
3
Cycle sequencing
4
One cycle of dye terminator cycle sequencing
5
one-color/four-lane method such as radioactive
sequencing
Four-color/one-lane fluorescent sequencing
6
(No Transcript)
7
Spectral overlap of the dyes in the four virtual
filters (vertical gray bars).
Example of a matrix
8
Sequence data obtained from a plasmid with BigDye
terminators.