SPIE lezing

1
PicoFill-SystemsOutlicensing Coverslip-Filling
of Wells on MicroArrays

• Requirements for Adequate Coverslip Filling
• Substrate Material coverslip- and chip surfaces
  should be rigid, smooth and hydrophylic allowing
  complete filling of wells and good bonding for
  complete sealing of wells Adequate materials
  glass, silicon nitride, polymethyl methacrylate,
  metal.
• Material thickness coverslips and chips may be
  as thin as 300 micron allowing monitoring in a
  ScanArray. Thicker material is preferred because
  it is stronger and more rigid.
• Pipetting of Sample Liquid a pipetting needle
  adds nanoliter-microliter amounts of liquid onto
  the chip in front of the array. Adequate liquids
  are all aqueous solutions, DMSO, alcohols,
  glycerol.
• Automated sliding device one actuator presses
  the coverslip onto the chip while a second
  actuator moves the coverslip across the chip to
  slide the liquid into the wells followed by
  well-sealing.
• Removal of excess sample after filling, some of
  the excess liquid has to be removed from the edge
  of the coverslip.

2
Application enzyme assays
Filling wells using coverslip
Spraying enzyme solutions 20 wt sugars
Sample liquid
Enzyme spots
Storage of chips for months
Shipping chips to end-users
Coverslip
Monitoring of enzyme assays using a ScanArray or
underneath Microscope objective