Bacteriological Terms

1
Bacteriological Terms

• Agar
• Aseptic Technique
• Bacteriology
• Culture
• Colony

• Solidifying agent
• Method of transfer
• Study of Bacteria
• Bacteria and material they are grown on
• A visible growth of bacteria

2
Bacteriological Terms

• Chromogenic
• Disinfectant
• Pure Colony
• Sterile
• Slant
• Fixing

• Producing a pigment (color)
• Substance that keeps bacteria from growing
• 1 kind of bacteria only
• No bacterial growth
• Test tube with agar
• Passing slide through flame

3
Bacteriological Terms

• Inoculum
• Morphology
• Incubate
• Media
• Plate
• Inoculate

• Substance being transferred
• Study of shape and form
• Grow at ideal temp.
• Substance bacteria is grown on
• Petri dish with agar
• Add bacteria to medium

4
Medium Prerequisites

• Nutrients
• Moisture
• Proper pH
• Sterility

5
Components of Nutrient Broth

• Distilled water
• Peptone
• Meat extract

6
Components of Nutrient Agar

• Distilled water
• Peptone
• Meat extract
• Agar

7
Compare and Contrast

• Derived and natural media
• Natural media come from plant and animal tissues
• Derived are synthetic
• Both can be used to grow bacteria but
• We used only derived

8
Reasons for Fixing

• Stick bacteria to slide
• Kills dangerous pathogens

9
Techniques

• Hanging drop
• Simple stain
• Negative stain
• Gram stain
• Streak plate

• Check for motility
• Blue bacteria white background
• Red background white bacteria
• bacteria purple - bacteria pink
• Isolate colonies