MATRIPOR PLUSTM Innovation in the Electroporative techniques applied to Aesthetic Medicine

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MATRIPOR PLUSTMInnovation in the Electroporative
techniques applied to Aesthetic Medicine

• Romulo Mene
• M.D. - Plastic Surgery - Brasil
• Andrea Fratter
• Pharm. D Cosmetic and Nutriceutical Science
• Venice

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Vessels dedicated to transport of water and
ions into cells

• 2003
• NOBEL PRIZE FOR CHEMISTRY
• Dr. Peter Agre
• Dr. Roderick MacKinnon

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BIO-PHYSICAL CONCEPTS

• Eucariotic cell is sorrounded by a lipidic
  bi-layer which separate the aqueous place of the
  cell (citosol) from the outer keeping the
  maintenance of the biochemical and
  electro-physiological paramethers (omeostasis)
• This double lipidic bi-layer is constituted by
  phospholipids, cholesterol and its esthers and
  Polynsathurated Fatty Acids which permit to
  maintaine the cell membrane fluidity
• Embedded in the lipidic bi-layer are several
  proteins with different functions like receptors,
  hydrophilic channels, biochemical signal
  transduction and electrochemical trans-membrane
  streams regulation

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SELECTIVITY OF THE TRANS-MEMBRANE MOLECULAR
GRADIENT

• Through the lipidic cell membrane the molecules
  can diffuse according to strictly defined
  biochemical conditions and electro-physiological
  gradients
• Pore proteins embedded in the bi-layers have
  the function to permit the passage of small
  hydrophilic molecules and ions through the
  lipidic membrane that couldnt be crossed
  otherwise
• This pore proteins or hydrophilic channels
  are constitutevely present in the bi-layers, but
  recently has been discovered that these
  channels can be switched on by particular
  physical impulses applied on the cell

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TRANS-CUTANEOUS MOLECULAR ABSORPTION MAIN
PRINCIPLES

• The main chemico-physical and mechanical obstacle
  that opposes to the penetration of molecules
  through the skin is the horny layer intended as a
  structure composed of horny cells and lipidic
  matrix in which are embedded (average thickness
  15 mm)

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Horny layer
basal layer
Giunzione D-E.
Fibroblast
Connectiv fibers
Fat cells
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MATRIPOR PLUS INNOVATIVE ELECTROPORATIVE
TECHNIQUES

• Electroporation is a phenomenon induced by the
  application on a biological surface of an
  electrical impulse capable to generate
  electro-pores inside the lipidic bi-layer of
  the cell membrane
• This electrical impulse associated to a low
  frequency wave is capable to induce a
  trans-membrane electrical potential ranging from
  0,5 to 1,5 V
• If the aforesaid electrical impulse is applied
  with a modulate frequency and with the features
  of a discontinous electrical stream
• ELECTROPORATIVE PHENOMENON ASSUMES PECULIAR
  FEATURES AND IS CAPABLE TO HIGHLY ENHANCE THE
  ACTIVE COMPOUNDS PENETRATION THROUGH THE SKIN

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MATRIPOR PLUS ACTIVATION STEPS

• STEPS OF MATRIPOR PLUS
• 1) IONIZATION OF THE SUBSTANCE the
  elastopulsated current meets the molecule then it
  ionises it and makes it active ready for
  transport
• 2) TRANSMISSION the ionised molecule is
  transferred to the optimal depth
• 3) ELECTROPORATION around 12 13 minutes,
  electropores are created. The molecules diffuse
  from one cell to the other by osmosis

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MATRIPOR PLUS BIO-PHYSICAL EFFECTS

• After application of the electrical pulsated
  impulse occur
• Dramatic enhancement of epidermal layer
  permeability toward hydrophylic exogenous
  molecules even with high molecular weight and
  dimensions
• Time-depending openess at cellular level of
  hydrophilic pores in the membrane bi-layer
  capable to lead the molecules inside the cell and
  then diffuse from a cell to another by mean of
  the electro-osmotic gradient generated

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MATRIPOR PLUS

• YOU CAN REALIZE MESOTHERAPY WITHOUT APPLYING ANY
  NEEDLES

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CELL WIDE ANGLE
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FEATURES OF THE ACTIVE MOLECULES
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MATRIPOR PLUS ADVANCED ELECTROPORATIONMain
treatments in Aesthetic Medicine

• Cellulitis
• Skin aging
• Fine Wrinkles (muscle contraction)
• Melanic pigmentations
• Skin metabolism boosting
• Trophism of veins and lymphatic vessels
• Metabolic localized improvement in course of
  weight loss diet regimens
• Muscles tonification
• Pain relief

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MATRIPOR PLUS PECUALIRITIES

• Ionic activation of the molecules to transfer
  in the ionization chamber thanks to the
  interaction between the elasto-pulsated electric
  impulse and the molecules
• Deep absorption of the active molecules (8-10 cm)
  even of high weight and dimensions (GAG, Soluble
  Collagen, peptides)
• Hydrophlilic electro-pores induction after
  several minutes from the application of the
  electrical impules on the skin surface and
  creation of an electro-osmotic gradient of the
  molecules delivered (transfer of the activated
  molecules from a cell to another)

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IONIZATION CHAMBER
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CELLULITIS
BEFORE TREATMENT
AFTER TREATMENT
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CELLULITIS
BEFORE TREATMENT
AFTER TREATMENT
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THANK YOU FOR THE ATTENTION