Regulation by the Heme Cofactor in Proteins

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Regulation by the Heme Cofactor in Proteins
Robert W. Clark Visiting Assistant
Professor Chemistry clarkr_at_hope.edu
B.S., Central Michigan University, 1999 M.S.,
University of Wisconsin-Madison, 2001 Ph.D.,
University of Wisconsin-Madison, 2005
Heme-specific inhibition in a Ca2-regulated K
channel?
Clark Research Group Spring 2006
Areas of expertise Bioinorganic Chemistry,
Mechanistic Studies of Heme Proteins,
Spectroscopic Studies of Metalloporphyrins

• Roles of Heme in Proteins
• O2 storage transport
• electron transfer
• catalysis
• regulation of protein function

Representative publications Clark, R.W., Lanz,
N. D., Lee, A. J., Kerby, R. L., Roberts, G. P.,
and Burstyn, J. N. (2006) Unexpected NO-Dependent
DNA Binding by the CooA Homolog from
Carboxydothermus hydrogenoformans, Proc. Natl.
Acad. Sci. USA 103, 891-896. Clark, R. W., Youn,
H., Lee, A. J., Roberts, G. P., and Burstyn, J.
N. DNA Binding by an Imidazole-Sensing CooA
Variant is Dependent on the Heme Redox State.
(manuscript in preparation) Pinkert, J. P.,
Clark, R. W. and Burstyn, J. N. Modeling Proline
Ligation in the Heme-Dependent CO Sensor, CooA,
Using Small Molecule Analogs (manuscript
submitted to J. Biol. Inorg. Chem.) Clark, R.
W., Youn, H., Parks, R. B., Cherney, M. M.,
Roberts, G. P., and Burstyn, J. N. (2004)
Investigation of the Role of the N-Terminal
Proline, the Distal Heme Ligand in the CO Sensor
CooA, Biochemistry 43, 14149-14160.
Transcriptional regulation by the CO sensor from
R. rubrum