Molecular Biology

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Molecular Biology

• Part IV Eukaryotes 4
• I. An Introduction to the Molecular Basis of
  Cancer

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Karp 1999 Cell Molecular Biology 2nd ed.
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normal human karyotype
human cancer aneuploidy
Marx, J. 2002 Science
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Karp 1999 Cell Molecular Biology
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Lewin 2000 Genes VII
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Lewin 2000 Genes VII
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Experiment by Stehelin, Bishop, Dulbecco 1976
Figure based on Bishop, 1982 Scientific American
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Watson et al. 1987 Mol. Bio. of the Gene 4th ed.
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Watson et al. 1987 Mol. Bio. of the Gene 4th ed.
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oncogenes can also be activated WITHOUT VIRUSES
Karp 1999 Cell Molecular Biology 2nd ed.
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Lewin 2000 Genes VII
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Karp 1999 Cell Molecular Biology 2nd ed.
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the human kinome
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Lewin 2000 Genes VII
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Watson et al. 1992 Recombinant DNA 2nd ed.
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Watson et al. 1992 Recombinant DNA 2nd ed.
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Karp 1999 Cell Molecular Biology 2nd ed.
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Karp 1999 Cell Molecular Biology 2nd ed.
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Karp 1999 Cell Molecular Biology 2nd ed.
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E2F transcription factor required for cell to
enter S phase RB protein binds E2F, preventing
cell from entering S (and RB E2F represses
other genes)
cyclin cyclin-dependent kinase (cdk) can
phosphorylate RB, causing it to release E2F,
which then binds to DNA and activates S phase
genes
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recessive mutation one functional allele
produces RB, which binds E2F and holds cell in Go
--- -- no functional alleles --gt no RB --gt E2F
activates S-phase
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What are some of the mutations that occur here?
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Karp 1999 Cell Molecular Biology 2nd ed.
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Karp 1999 Cell Molecular Biology 2nd ed.
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McKinnell et al. 1998 The Biological Basis of
Cancer