Operon structure

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Operon structure
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Either strand of a DNA molecule may be used as a
template, but transcription always reads the 3
to 5 strand relative to the direction of RNA
polymerase movement.
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Initiation--Transcription begins by the assembly
of the RNA polymerase holoenzyme on a promoter
region.
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S
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Orientation of promoter elements specifies the
direction of transcription
-35
-10
prokaryote
CAAT
TATA
GC
eukaryote
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The Transcription Bubble
Figure 26.8
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Figure 26.16
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rho-independent terminator
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Almost all introns begin with GUand end with
AG
Region that is removed from the RNA
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DNA
pre-RNA
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Removal of introns by the spliceosome
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