DNA EXTRACTION LAB

1
DNA EXTRACTION LAB

• LAB SAFETY INSTRUCTIONS

2
PROCEDURE

1. Get 2 cups from the front table.
2. Mark one cup Cheek with your initials
3. Mark the other cup Soap with your initials
4. Get 10 mL of the salt solution from the front
   table.
5. When everyone has their solution, we will all
   swish it in our mouths vigorously for 30 seconds.
   Ill be timing you!

3
PROCEDURE

1. After swishing, spit the solution back into your
   Cheek cup.
2. Get 5 mL of soap solution in your Soap cup from
   the front table.
3. Pour your Cheek solution into the Soap cup.
4. Gently mix this solution for 2-3 minutes! Ill
   be timing you!

4
PROCEDURE

1. Tilt the Soap cup and pour 10 mL of isopropyl
   alcohol down the side of the tube. DO NOT
   MIX!!!!
2. Let the tube stand for 1-2 minutes! Time it!
3. The white clump that you see is YOUR DNA!!!!
4. Write your reflection!

5
SAFETY!!!

• ALWAYS WEAR GOGGLES!!!
• Do NOT spit anywhere except YOUR cup!
• Be careful when measuring the solutions.
• Limit spillage!
• Wash your hands!
• ANY horseplay will result in a ZERO for the lab
  and a visit to your administrator!

6
REFLECTIONAnswer the questions in a paragraph
form

• What did the salt solution do?
• What did the soap do?
• Why would scientists want to isolate human DNA?
• How is DNA extraction useful for the future
  (medicine, crime work, etc)?