Prsentation PowerPoint

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Zendropper
CYTOGENETIC AUTOMATION Automated cell
distributor on standard microscopic glass side
for karyotyping and hybridization in situ
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Why the Zendropper?

• Automatisation of a very sensitive step
• High reproducibility of distribution from one
  patient to another and from one slide to another
• No inter-technician variations
• Homogeneisation and reliability of distribution
• Samples sparing
• Time saving and rapid execution

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Why the Zendropper?

• Optimization of laboratory tasks
• System using a software allowing traceability of
  cells dispersion (bar-coded slides session
  printing)
• Easier laboratory accreditation
• Better valorization of lab technician work
• Environmental factors under control ( T,
  humidity, local aeration) with Water Layer
  methodology.

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Zendropper methodologies
Cells preparation
Distribution on slide Hybridization in situ
Distribution on slide Caryotype
Double cells spot on same slide
Water Layer Methodology
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1. Karyotype procedure

• High automatic distribution capacity
• Homogeneous cells distribution
• 8 patients with 1 to 5 slides per patient (40
  slides on 1 rack)
• Timing (dispensing safety 100 )
• /- 25 for 40 slides
• ? Significant time saving
• Protection of technicians not exposed to
  methanol and acetic acid emanation from Carroy
  solution.

Distribution on slide ZenDropper
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Karyotype Water Layer technology
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Distribution of a water layer on each slide
Ejection of sample on the slide
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Needle containing sample is placed just above
the slide
Aspiration of residual water on slide
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Creation of a central crater in the water layer
due to Carroy solution evaporation
Slide ready for Banding step
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Hybridation in situ
Environment preparation T , Hygrometry
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Distribution of cell spot I
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Distribution of cell spot II
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Slide ready for next step
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Advantages of water layer technology
Karyotype Water Layer technology

• Improved mitosis thanks to contact water acetic
  acid/methanol mix on slide surface
• ? Chromosomes more spread
• ? Banding (R or G) with better quality
• Creation of a micro-environnement ? The
  distribution will always be done with same
  hygrometric conditions independently of climatic
  conditions

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Zendropper process
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Zendropper process
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Zendropper process validation
Comparative study Manual gtlt Zendropper CERBA
Pasteur - Paris (Genetics departement - More
than 32.000 samples with Zendropper since March
2007)

Contamination Protocol Cells pellets of 240
patients have been distributed on routine
microscopic slide alterning male/female
patient Results No chromosome Y observed on
female samples and confirmed by Quinacrine test
(100 mitosis per patient) Conclusion No
contamination between patients
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Zendropper process validation

• Quality of mitosis
• Less crossing of chromosomes with Zendropper
• Quantity of mitosis
• More reproducible and homogenous dispersion of
  cells in mitosis with Zendropper
• Quality of  Banding 
• High level of homogeneity from slide to slide
  with Zendropper
• Reading
• Easier with Zendropper due to quality of
  dispersion
• Very efficient particularly for metaphasis
  research
• Lab technicians reactions
• Easier and fast method than manual
• Less exposition to toxic vapours
• No more difficult work due to work position on
  bench or with a glove box.

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Technical Characteristics

• Dimensions 60x60x80 cm
• Weight 62 kg
• Rack with slides 8 patient X max 5 slides
• Tube holder for samples 8
• Extra washing needle solution 2 positions
• 2 needles
• Needle 1 - Mixing, aspiration and distribution
  of samples distribution of water
• Needle 2 Aspiration of residual water

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Technical characteristics
Zendropper system inclus

• Automate
• PC windows Xp
• Software for operations
• Software for setting up options of user and
  instrument calibrations (Ztoolbox)
• Printer
• 1 rack of 40 slides
• Option Bar code reader

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Zendropper Software

• Zendropper software Session preparation number
  of samples, number of slide/patient (codebar
  available) , print of session with information
  for tracability
• Ztoolbox Technical tool for maintenance and
  instrument set up in order to obtain high
  homogeneous cells dispersion conditions (Humidity
  and T)

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Zendropper maintenance

• Intensive rinsing procedure at start and end of
  the day
• Regular replacement of needles and tubing due to
  intense corrosion of solution

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