Review Session

1

• Review Session
• March 6 (Tuesday) at 600 pm
• Ramy (Ramaley Biology) Building N1B23

2
Three unifying principle for preinitiation
complex formation

• It always starts with an assembly factor that
  recognizes a specific binding site in the
  promoter
• TATA-containing class II promoters TBP
• TATA-less class II promoters TAFs or upstream
  element binding factors such as Sp1
• Class I promoter UBF
• Class III promterTFIIIC (TFIIIA)

3
2) TBP is always involved in the assembly
process, playing an organizing role in
preinitiation complex formation. 3) The
specificity of TBP is governed by its associated
factors (TAFs)
4
(No Transcript)
5
Which of the following statements is correct?

1. TFIIF is a component of the RNA polymerase II
   holoenzyme
2. TFIID is a component of the RNA polymerase II
   holoenzyme
3. All TFII factors are components of the RNA
   polymerase II holoenzyme
4. None of the TFII factors is a component of the
   RNA polymerase II holoenzyme
5. None of the above statement is correct

D
6
Which of the following statements is correct?

1. TAFs (TBP associated proteins) are shared by some
   RNA polymerases
2. TAFs can function independently of TBP
3. TAFs and TFIID play different roles in
   transcription
4. TBP can not function in vivo without TAFs
5. None of the above

D