Cyanobacteria Oscillator

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Cyanobacteria Oscillator

• Harvard iGEM 2006
• Week 3
• Peng, David, Jeff, Hetmann

E. Coli
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Overall goal

• We aim to create a kaiABC reporter BioBrick
  which expresses a circadian rhythm in both E.
  coli and cyanobacteria.
• (We are still brainstorming ideas of what to do
  this week was review literature / grow strains /
  contact people)

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Blue biobricks cut site Green can be obtained
from cyanobacteria
Prof. Susan Golden and others have this
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• Weve obtained three strains of cyanobacteria
• From Eric Webb at Woods Hole WH8102, a marine
  strain
• Were probably not going to use it because its
  reputed to be very difficult to grow and modify.
• From Peter Weigele at MIT PCC7942 and PCC6803,
  both freshwater strains
• PCC6803 can metabolize glucose.
• PCC7942 is the most common strain weve
  encountered in the literature on cyanobacteria
  circadian oscillation.
• Both strains genomes have been sequenced.
• Both can be reliably transformed by exogenous DNA.

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Incubator

• We modified an incubator by installing a
  timer-controlled fluorescent light (32W 22W
  bulbs)
• The lights are set to stay on for 16 hours each
  day
• According to Peter Weigele, we should expect
  growth in 5-7 days (Tuesday-Thursday this week)

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Next steps

• Design primers for extracting KaiABC
• Learn protocols for transforming PCC7942 and
  PCC6803
• (Maybe) synthesis of kaiABC
• Waiting to hear back from
• Susan Golden at UT Austin
• Alexander van Oodenaarden at MIT
• Jeffrey Chabot