Barrier Policies and Procedures

1
Barrier Policies and Procedures

• Patrick Sharp, DVM
• UCLA School of Medicine
• Division of Laboratory Animal Medicine

2
Purpose

• Minimize pathogen exposure to animals held in the
  UCLA barrier facilities
• Mac Donald Research Laboratories (MRL)
• Public Health (B floor)
• The barrier is a community area
• The outlined practices are designed to reduce the
  risk of pathogens entering and spreading within
  the facility

3
Scope

• All investigators housing animals in the barrier
  and those sharing facilities, experimental
  equipment, laboratory space, and/or other such
  circumstances with investigators or individuals
  outside the barrier.

4
Consequences

• UCLA currently receives over 300 million in
  contract and grant awards, placing us second only
  to Harvard
• UCLA stands to lose significant resources
  financially and/or staff-wise due to an untoward
  pathogen outbreak
• Please follow the outlined policies

5
Overview

• Barrier Use
• Barrier entry
• Barrier practices
• Barrier exit
• Animal Research Committee (ARC) Concerns
• Animal Transport in Public Areas
• Animal Use in Public and Clinical Areas

6
Overview

• Ordering Animals at UCLA
• Exporting Animals at UCLA
• Transferring Animals at UCLA
• Sentinel Program
• Division of Laboratory Animal Medicine
• Technical Services
• Pharmacy
• Diagnostic Laboratory

7
Barrier Use

• Barrier entry
• Barrier practices
• Barrier exit

8
Barrier Entry

• Impacts four areas
• Scientific personnel
• Physical plant personnel
• Animals
• Equipment

9
Barrier Entry

• Gowns
• Booties
• Cap
• Mask
• Gloves
• Equipment

10
Barrier Entry

• Gowns
• Put on a new gown every time you enter the
  facility
• Throw the gown away if it touches the floor
• Additional gowns may be need to be worn in some
  rooms

11
Barrier Entry

• Booties
• CHS Foot bath
• Step into the foot bath while donning the booties
• MRL
• Spray an area outside the red area with
  disinfectant
• Step into this area donning the booties
• Wipe your feet in the sprayed area
• Additional booties may be need to be worn in some
  rooms

12
Gloves

• Put on gloves before handling animals
• Put on gloves before wiping down items which may
  be brought into the barrier
• Glove etiquette

13
Equipment

• Materials brought into the barrier must be
  contained within a plastic bag.
• Generously spray down the plastic bag contained
  items with the disinfectant
• Wipe the outside of the bag with your gloved
  hands
• Open the closed plastic bag ONLY in the cage
  change station.

14
Equipment

• Non-barrier hand carts do not enter the barrier
• Barrier hand carts do not leave the barrier
• Disinfect before each use
• Disinfect after each use

15
Animals

• Importing or exporting animals
• Dierk Biggs 51826 ltdbiggs_at_mednet.ucla.edugt
• Animal transfers
• Ken Jones 57140 ltkjones_at_mednet.ucla.edugt
• Cell lines and tumors passed in mice must be
  tested for murine pathogens
• Jerry Werk 56497 ltjwerk_at_mednet.ucla.edugt

16
Special Housing/Husbandry

• Biohazard
• Radioactive materials

17
Other

• Human cell lines
• Human tumors

18
Barrier Practices

• Keep your eyes open
• Ask questions
• Traffic
• Bag-up items BEFORE entering the facility
• Bag-up items BEFORE leaving an animal room
• Work from cleanest to dirtiest
• Minimize traffic within the facility

19
Barrier Practices

• Cage change station etiquette
• Turn on
• Wipe down the hood bottom AND sides
• Handle animals
• with tongs if possible, disinfecting between
  cages AND when finished
• gloved hands, disinfect between cages
• Bag up supplies/animals before leaving the room
• Wipe down the hood when finished bottom AND sides

20
Barrier Practices

• Animals
• Open cages within a cage change station or hood
• Disinfect gloved hands between cages
• Do not place animals on microisolator tops Wire
  tops are OK

21
Barrier Practices

• Animal Escapes
• Euthanize the animals
• Identify cages as Change Last so these cages
  can be changed prior to the sentinels
• Humane Animal Traps

22
Barrier Practices

• When exiting your animal room
• Follow the posted exit procedures
• All clothing put on immediately prior to entering
  the animal room
• Must be removed before exiting the room, and
• Disposed of in the room you are exiting

23
Barrier Practices

• Room lighting
• Euthanizing animals
• Clean cages
• Pets Rodents, reptiles, etc.

24
Exiting the Barrier

• Impacts four areas
• Scientific personnel
• Physical plant personnel
• Animals
• Equipment

25
Exiting the Barrier

• Personnel
• Do not wear barrier clothes outside the barrier
• This includes gloves and booties
• Plan on not returning to the barrier

26
Exiting the Barrier

• Animals exiting the barrier must be appropriately
  handled
• Covered by an opaque material (black garbage bag)
• Cardboard transport box

27
Exiting the Barrier

• Animals cannot be housed outside of the barrier
  facility over 12 hours without Animal Research
  Committee (ARC) approval
• Contact the ARC for approval
• 310.20.66308
• ltwww.oprs.ucla.edu/animalgt

28
Exiting the Barrier

• Animals leaving the barrier MUST NOT return to
  the barrier
• They MUST be placed in the Animal Return Room
• Matthew Dang 310.82.54080 ltmdang_at_mednet.ucla.edu
  gt
• Ken Jones 310.82.57140
• ltkjones_at_mednet.ucla.edugt
  

29
Animal Research Committee (ARC)

• Location 2107 Ueberroth Building
• Phone [phone]
• Fax [phone]
• Website www.oprs.ucla.edu/animal
• Animal Transport Through Clinical and Public
  Areas
• Animal Use in Clinical and Public Areas

30
Animal Transport Through Clinical and Public Areas

• Rodents must be transported in covered shoebox
  cages or transport boxes
• Double bag dead animals in an opaque durable
  plastic bag of appropriate size.

31
Animal Transport Through Clinical and Public Areas

• Transporting animals in vehicles not specifically
  designated for animal transportation (e.g.
  personal or rented vehicles) is strongly
  discouraged
• Please contact the Division of Laboratory Animal
  Medicine (x57281) for details concerning animal
  transport in vehicles

32
Animal Transport Through Clinical and Public Areas

• Service elevators must be used when available
• This includes the transportation of empty
  carriers, restraint devices, transport cages,
  shoebox cages, etc.

33
Animal Use in Clinical and Public Areas

• Please follow the Animal Transport Through
  Clinical and Public Areas guidelines
• Patients and non-essential employees must not be
  present
• Employees participating in the procedure should
  be listed on the Animal Research Committee
  approved protocol

34
Animal Use in Clinical and Public Areas

• Door and interior window shades in the procedure
  room should be closed to avoid view and/or
  intrusion
• Countertops, floors, equipment, and other areas
  of the procedure room where animals were used
  should be thoroughly cleaned and disinfected
  after procedures have been performed

35
Animal Use in Clinical and Public Areas

• Disposal of animal waste, trash, and debris shall
  comply with applicable federal, state, and local
  laws relating to protection of the environment
  and pollution control

36
Ordering Animals at UCLA

• Animal sources
• Order Online At www.dlam.ucla.edu/
• Animals
• Online Animal Order Form
• Importing
• Domestic
• International
• Transferring
• Exporting
• Domestic
• International

37
Ordering Animals at UCLA

• There are essentially three sources of research
  rodents at UCLA
• Approved Sources,
• Unapproved Sources, and
• Internal Transfer

38
Ordering Animals at UCLA

• Approved Sources include
• Vendors
• Harlan
• Taconic, and others
• Other organizations providing us regular sentinel
  reports
• Contact Tensie Palmer
• Orders MUST be received prior to 4 PM Tuesday
• Phone [phone]
• E.mail lttpalmer_at_mednet.ucla.edugt
• Animals are available the Monday after their
  arrival

39
Ordering Animals at UCLA

• Unapproved Sources include any organization not
  providing UCLA regular sentinel information
• Universities
• Government
• Others
• Contact Jamie Tran
• Phone [phone]
• E.mail ltjtran_at_mednet.ucla.edugt

40
Ordering Animals at UCLA

• Ordering from Unapproved Sources
• Give at least three weeks notice
• Complete an Animal Shipping Record
• ltwww.dlam.ucla.edugt
• Click on Forms and Records
• Select Animal Shipping Record
• Online submission
• Download the document

41
Ordering Animals at UCLA

• Ordering from Unapproved Sources
• Once a shipment has been approved it has one of
  three destinations
• Special Quarantine
• Deferred Quarantine
• Biocontainment

42
Ordering Animals at UCLA

• Special Quarantine
• At least 6 weeks
• Individual testing
• Sentinel testing

43
Ordering Animals at UCLA

• Deferred Quarantine
• Animals must appear to be clean based on the
  sentinel information provided by the source
  institution
• All animals used Monday 8 AM following their
  arrival at UCLA
• Testing of some/all sacrificed animals

44
Ordering Animals at UCLA

• Biocontainment
• Short term
• Source of infection of clean animals housed in
  barrier and defined flora facilities
• Requires rederivation to enter the clean
  facilities
• Cesarean
• Embryo Transfer

45
International Imports

• Must meet these agency requirements
• US Treasury
• US Customs
• US Department of Agriculture (USDA)
• Animal and Plant Health Inspection Service
  (APHIS)
• US Department of Health and Human Services
• Centers for Disease Control and Prevention (CDC)

46
Transferring Animals at UCLA

• Internal Transfer
• Complete an Animal Transfer Record
• ltwww.dlam.ucla.edugt
• Click on Forms and Records
• Select Animal Transfer Record
• Ken Jones
• Phone [phone]
• CHS 1V-203
• Document animal traffic between rooms,
  facilities, investigators, and protocol numbers
• Six week quarantine
• Adds validity to the Sentinel Program

47
Exporting Animals From UCLA

• Complete an Animal Shipping Record
• ltwww.dlam.ucla.edugt
• Click on Forms and Records
• Select Animal Shipping Record
• Online submission
• Download the document
• The Division will provide the receiving
  institution with the previous 9 months of
  sentinel information
• Please DO NOT provide the sentinel information to
  reduce confusion

48
Exporting Animals From UCLA

• The receiving institution may do one of the
  following
• Accept the shipment
• Immediate export
• Delayed (space availability)
• Reject the shipment
• Health
• Space availability

49
Exporting Animals From UCLA

• Upon accepting the shipment the receiving
  institution will provide the Division with a
  shipping address
• The animals will need to be inspected with in 10
  days of departure, and a USDA Health Certificate
  may need to be issued

50
Exporting Animals From UCLA

• The UCLA investigator is currently responsible
  for making the shipping arrangements
• Animals are to be shipped on a Monday, Tuesday,
  or Wednesday of a normal week

51
Exporting Animals From UCLA

• Three pieces of information must be collected
  from the transporting agency
• Courier
• Tracking number
• Expected date of arrival
• The Division will provide this shipping
  information to the Receiving Institution

52
Exporting Animals From UCLA

• Ship ONLY the number of animals indicated on the
  Animal Shipping Record

53
International Exports

• Must meet these requirements
• USDA
• Health Inspection Valid for 10 days
• Endorsed Health Certificate Valid for 30 days
• Country of Destination
• International Air Transport Association

54
Sentinel Program

• High-risk animals
• Monitoring
• Gross lesions
• Microscopic lesions
• Serology ELISA, IFA, HAI
• Molecular

55
The UCLA Rodent Sentinel Program

• Animals housed on a sample of dirty bedding from
  all other rodents on a given rack
• Research animals must not be moved from one rack
  to another
• Marked with a Sentinel sticker on the cage card
• Do not use or euthanize these animals

56
The UCLA Rodent Sentinel Program

• The animals are necropsied and blood is collected
  for serology
• If gross lesions are observed then histopathology
  is performed
• All serologies from a given sentinel quarter are
  sent to the same diagnostic laboratory for
  consistency

57
The UCLA Rodent Sentinel Program

• Ectromelia
• Epizootic Diarrhea of Infant Mice (EDIM)
• Lymphocytic Choriomeningitis (LCM)
• Minute Virus of Mice (MVM)
• Mouse Hepatitis Virus (MHV)
• Mouse Parvovirus

• Mycoplasma pulmonis
• Pneumonia Virus of Mice (PVM)
• Reovirus
• Sendai
• Theilers Murine Encephalomyelitis (TMEM)
• Endoparasites Pinworms
• Ectoparasites Mites

58
The UCLA Rodent Sentinel Program

• Additional serology panels are available
• Cost/Sample requirements
• Contact Jerry Werk
• Phone [phone]
• E.mail ltjwerk_at_mednet.ucla.edugt

59
Division of Laboratory Animal Medicine Services

• Training
• Technical services
• Pharmaceutical and research supplies
• Division website www.dlam.ucla.edu
• Pathology and research support services

60
Division of Laboratory Animal Medicine

• Training FREE
• Technical service
• Fee for service basis
• 6.00/15 minutes (AHT)

61
Division of Laboratory Animal Medicine

• Pharmaceuticals, equipment, and supplies for
  animal research may be purchased through the
  Divisions Clinic
• Controlled substances can be purchased from the
  hospital pharmacy
• Contact Talia Acosta
• Phone [phone]
• E.mail lttacosta_at_mednet.ucla.edugt

62
Division of Laboratory Animal Medicine

• Website
• ltwww.dlam.ucla.edugt
• Let us know if something should be added

63
Division of Laboratory Animal Medicine

• Necropsy
• Serology
• Bacteriology
• Parasitology
• Cell line and tumor testing (IMPACT)
• Clinical Chemistry

• Hematology (CBC)
• Urinalysis
• Histopathology
• Characterizing KO/Tg animals

64
Division of Laboratory Animal Medicine Veterinary
Faculty

• Timothy Lawson, DVM
• 310.82.53178 lttlawson_at_mednet.ucla.edugt
• Director, Division of Laboratory Animal Medicine
• Diplomate, American College of Laboratory Animal
  Medicine

65
Division of Laboratory Animal Medicine Veterinary
Faculty

• Patrick Sharp, DVM
• 310.82.52776 ltpsharp_at_mednet.ucla.edugt
• Associate Director, Division of Laboratory Animal
  Medicine
• Diplomate, American College of Laboratory Animal
  Medicine

66
Division of Laboratory Animal Medicine Veterinary
Faculty

• Rebecca Schwiebert, DVM, PhD
• Phone [phone]
• E.mail rschwiebert_at_mednet.ucla.edu
• Director, Primate, Minor Species, and Clinical
  Training Services
• Diplomate, American College of Laboratory Animal
  Medicine

67
Division of Laboratory Animal Medicine Veterinary
Faculty

• JanLee Jensen, DVM
• Phone [phone]
• E.mail jjensen_at_mednet.ucla.edu
• Director, Surgical Services
• Diplomate, American College of Veterinary
  Internal Medicine

68
Division of Laboratory Animal Medicine Veterinary
Staff

• Gregory Lawson, DVM, PhD
• Phone [phone]
• E.mail glawson_at_mednet.ucla.edu
• Director, Diagnostic and Rodent Services
• Eligible, American College of Veterinary Pathology