Title: Giving a Talk
1Giving a Talk
- Dr Alan Wheals
- University of Bath
- October 31st 2007
2Power corrupts. PowerPoint corrupts absolutely
- Edward Tufte
3Topics
- Introduction
- Types of talks
- Audience
- Speaking
- Slide content
- Standard Plan
- PPT Practicalities
- Fonts, Figures, Tables, Style
- Finishing
4Introduction
- It is an essential skill for professionals
- Few of us are natural public speakers
- I will talk about things to avoid
- Practice makes perfect
- You need to be able to tell a story
- For scientists, content is always more important
than style, BUT - Poor style and presentation may obscure content
5Types of talks you may give
- Seminar (assessed for MRes)
- Project talk (assessed for MRes)
- PhD progress report
- PhD interview (as part of a)
- Post-Doc interview (as part of a)
- Conference talk
- Faculty position talk
6Other types of talks you may give
- School (eg as an alumnus/a)
- A non-Science department here
- Job Interview Panel
- Public
- Funding body
7Know your audience
- Adjust content for every occasion
- BBSRC require a research project summary
intelligible to a 14-year old - Outreach justifies expenditure on Science
- Science Café
- Researcher in Residence
- Elevator Pitch?
- Can you explain your research topic in one
minute? - Try it on the adjacent person and vice versa
- For the receiver was the talk good OK not so
good?
8Speaking 1
- Coping with nerves
- Rehearse until you are confident
- Concentrate on your story
- Check up on any weak bits
- Read from slide? Boring
- Read from notes/cards? Less boring
- Use slide as a reminder and explain in your own
words. Best - Keep to time. You may get cut!
- Less is more. Concentrate on essentials
9Speaking 2
- Slowly
- Talks with audiences go faster (panic)
- Clearly
- Enunciate (most of the audience might be
non-native speakers) - Breathing
- Ends of sentences
- Eye contact
- Volume
- Use a microphone if available
- If a radio-mike, where does it go?
10Highlighting
- Pointer
- Hand?
- Laser
- Stick
- Mouse
- Visible all the time
- Drawing
- Mouse options on Control Panel
- Arrow size or alternatives
- Trails
11Slide content
- The audience needs time to
- read the words
- understand the figures and tables
- interpret the slide
- You must explain the slide and not expect the
audience to work it out - Figures can contain a complex story but you must
tell it
Sample size? What is an adult? When was time
zero (or one)?
http//www.prcdc.org/summaries/aidsinafrica/aidsin
africa.html
12Slide content
- Check out the venue (or screen) size
- Some professional societies recommend no more
than 6 text lines - Do not overload the slide
- It is usual to make only one point per slide
- One slide per minute would be an absolute upper
limit and half that would be more common - Movies are great but ensure that the software is
on the computer or your flashdrive
13Standard Plan
- Plan of talk
- Introduction/background
- What you were trying to do the problem
- Why you were trying to do it - importance
- How you did it methods / approach
- What did you discover - results
- Say how important it was conclusions
- Where you go next future work
- Thanks (acknowledgments) to people and funders
14Practicalities
- A talk is different from a report or poster
- Dont just paste from Word
- Use animation (appropriately)
- Learn from others (good and bad)
- Focus on the essentials
- Edit large tables down to key points
- Use diagrams (that can be borrowed from the
internet, with attribution) - Use the whole slide
- Use slide numbers to know where you are
15Slide set-up
- Master slide
- View (Master then Slide master)
- Background, font style, colour, size
- Logo
- Colour schemes
- Design templates
16Fonts type and size
- Times New Roman 24 20 16 12 8
- Verdana 24 20 16 12 8
- Arial 24 20 16 12 8
- Tahoma 24 20 16 12 8
- Comic sans MS 24 20 16 12 8
- Lucida Sans 24 20 16 12 8
- Dont mix fonts
- Use sans serif
- Use the Master slide
17Fonts type and size
- A quick brown fox jumped over the lazy dogs
- A quick brown fox jumped over the lazy dogs
- A quick brown fox jumped over the lazy dogs
- A quick brown fox jumped over the lazy dogs
- A quick brown fox jumped over the lazy dogs
- A quick brown fox jumped over the lazy dogs
- All 26 font
18Colour
- Red
- Orange
- Yellow
- Green
- Blue
- Indigo
- Violet
- White
19Colour
- Red
- Orange
- Yellow
- Green
- Blue
- Indigo
- Violet
- White
20Colour
- Red
- Orange
- Yellow
- Green Blue
- Indigo
- Violet
- White
21Colour
- Calm colours are best
- One colour is best
- Do not overuse
- and its impact will be greater
- Do not use templates
- Use logos sparingly
- Colours will vary with location set up
- Use distinct primary colours
22Acknowledgements
- Thanks cost nothing
- The lab
- Supervisors
- Sponsors
- (University of Bath for funding)
- My mother without whom
23Some examples to criticise!
24Average OTA uptake in ng/day, MPP, 50th
percentile (mean case)(Wolff et al., 1999)
25- The history of the motor car
- 1920-1929
- Henry Ford
26Wild Strains - O2 Results
27Phylogeny
- Tree based on sequences of rDNA cistrons,
particularly the D1-D2 region of the Intergenic
Transcribed Spacer (ITS)
28The prion protein
- 25kDa Protein
- First isolated in 1982
- Consists of several domains
Toxic domain
1 23 51 90
254
Octameric repeats
Copper Binding
GPI Anchor
Signal Peptide
29Cell Tracking
37 hours/30min IN Cell Analyzer 1000 37C/5 CO2
30Cell tracking
time
31History of opium II
- In 16th century, addiction and withdrawal
symptoms were reported - Godfreys cordial
- In mid18th century ?opium war between China and
western countries.
32Background
- Shigella spp. are a specialised clade of E.coli
responsible for shigellosis in humans, a disease
characterized by the destruction of colonic
mucosa induced upon bacterial invasion - Homologous genes from closely related organisms
such as E.coli allow an examination of the
mechanisms of evolution (Fukushima et al. 2002) - The rate of nucleotide modification through
synonymous changes allows us to compare closely
related species - Evolution at silent sites has been used to
estimate the rate of selectively neutral
processes inferring differences and relationships
in divergence times of genes and genomes allowing
the investigation of specific mutational
modifications - In addition to codon and gene specific effects on
synonymous site substitution rates, genes located
near the origin of replication undergo a lower
rate of substitution than genes situated closer
to the terminus - Marais (2003) suggested that the main cause is an
increase in rates of transitions and
transversions
33GC Enrichment
- All data is normalised for codon preferences
(substitution type / total number of substitution
bases available) and for differences between
windows e.g. base composition length (each sub
type / total subs for that window) - Allows better direct comparison between different
species - Any score greater than 1 is GC enriching and any
score less than 1 is AT enriching
34Staphylocuccus aureusA Super Bug
- Reduction in S. aureus infections a priority in
NHS hospitals - Considerable efforts in infection control
- Washing hands is probably not enough
- Alcogel used in hand washing may be exacerbating
the spread of Clostridium difficile
35Infection associated?
total IgG
- 9/11 infection associated
36Introduction Lipid transfer proteins
- Bind and transfer lipids in vitro
- For a few there is evidence for in vivo transfer
- Distinct lipid transfer proteins with different
lipid affinity - One family of lipid-
- transfer protein StAR-
- related lipid transfer
- (START) domain
- protein
Alpy, F. et al., J. Cell Sci. 2005 1182791-2801
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38Finally
- Take your PPT in multiple formats
- Will the audience remember anything?
- Will the audience remember your name?
- Will you be invited to speak elsewhere?
- Your career may be influenced by it!
39Thanks for listening