Study Objectives Presentation Outline

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Study Objectives Presentation Outline

• Hood River STH Status

1.) Characterize summer run (SR) and winter run
(WR) life Histories,
2.) Hatchery practice and status
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Columbia
River
Hood River Basin 35 km upstream of Bonneville Dam
Hood River
Punchbowl Falls
Miles
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Ecotype and Life History Descriptions
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Hood River Summer-Run (SR) spawners
Hood River Winter-Run (WR) Spawners
Smolts outmigrate early summer
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Chi-square Prob. lt 0.0001
total return
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Statistically Significant WR Fewer months in
the ocean
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Natural origin (threatened) populations require
Conservation measures
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Challenges of Identifying SR and WR

• Most SR return between late June and mid January
  

• Most WR return between February and early June

• But, overlap February to June

• Physical characteristics

• Body is thicker, more girth

• Body is long and lean bullet

• External coloration

• Sexual maturity relative to the time of year

Can Genetics Help Clarify Identity?
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Genetic Evaluation Objectives
1.
2.
If YES
3.
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Methods sampling and data quality
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Result Genetic Structure Analysis
- P lt 0.0001
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East Fork 2003
SR adult 2001
WR adult 2001
West Fork 2003
East Fork 2004
649
506
759
1000
539
SR adult 2002
992
602
WR adult 2002
West Fork 2004
Middle Fork 2003
Middle Fork 2004
- Bootstrap support shown at nodes
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Baseline Jackknife Assignment Juveniles

• Re-sampling Procedure WHICHRUN - (Banks and
  Eichert 1999)

• Compare individual multilocus genotype to those
  in baseline populations

• Assign population with highest probability of
  producing
• an individuals multilocus genotype

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Juvenile Jackknife Assignment Results
91 Overall
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Assigned WR
Assigned SR
LOD 0
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95 Confident Assigned WR
95 Confident Assigned SR
Ambiguous Zone
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Application Rapid Response Protocol

• All NOR fish evaluated as possible brood stock
  

• Assigned an Ecotype Origin Using Baseline

- 95 confident SR - 95 confident WR - Or if in
ambiguous LOD zone Unknown

• Unknown returned to River access to spawning
  grounds

• First Implemented in 2005

• 112 individuals evaluated

• 32 individuals assigned to SR brood stock

• 39 individuals assigned to WR brood stock

• Baseline updated for 2006 RTRR temporal
  variability

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Summary and Conclusions

• Hood River SR and WR steelhead Biologically
  Distinct

- Return time - Spatial separation Spawning
grounds - Sexual maturity

• Hood River SR and WR steelhead Genetically
  Distinct

- Return time - ? - Spatial separation Spawning
grounds SR in west fork, WR in middle east
forks

• Level of Genetic Difference Power of Assignment

• SR 82 correct assignment, and 77 (95 CI),
• WR 91 correct assignment, and 82 (95 CI)

- RTRR implementation adopted and ongoing
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Acknowledgements
- Bonneville Power Administration
Oregon Dept. of Fish and Wildlife

• Jim Gidley, Alexis Vaivoda
• Confederated Tribes of the
• Warm Springs Reservation
• ODFW biologists (Tyla Metzentine)

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