Cooling Stage for Light Microscope

1
Cooling Stage for Light Microscope

• BME 401
• Group 16
• Raj Venkata
• Donghoon Ryan Lee
• Tony Chen
• Mentors
• Dr. David Sept
• Dr. John Cooper

2
Overview

• Need for a Cooling Stage
• 2. Final Design Specifications
• 3. Design Analysis
• 4. Chosen Design Details
• 5. Design Visual
• 6. Control Flow Chart
• 7. Calculations
• 8. Safety Analysis
• 9. Parts Prices
• 10. Conclusions

3
Need for a Cooling Stage

• Temperature sensitive mutants
• Certain phenotypes only visible under low
  temperature
• No effective way to control condensation and
  temperature gradients

NR Adams, JR Oberle and J Cooper. The
Surveillance Mechanism of the Spindle Position
Checkpoint in Yeast. Journal of Cell Biology,
Vol. 153, No. 1, pg 159-168, April 2, 2001,
4
Design Specifications

• Temperature Control 10 C to ambient 1 C
• Rate of cooling 3 C / min
• Stress on stage lt 2 N
• Avoid Condensation
• Avoid Vibration
• Minimize Temperature Gradients
• Fit for Olympus BX52 microscope stage (17x14cm)
• Acceptable cost up to 2000

5
Air-Flow Cooling System Schematic
Cooling Chamber
Moisture Remover
Stage
Air Vent
Power Source
Thermocouple
Cold Air Source
Pump
Feedback
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Design Pugh Chart
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Design Details
Compressed air
Hot air exhaust
Cold air
http//www.exair.com/vortextube/vt_frmain.htm

• Cold Air Source Vortex Tube
• No moving parts
• Compact lightweight
• Temperature control 0 C to 25 C
• Interchangeable parts

8
Design Details
http//www.chem.agilent.com

• Moisture Remover Silica Gel
• Absorbs 40 of its own weight
• Saturation indicator
• Rechargeable

9
Design Details
http//www.chem.agilent.com

• Insulating Jacket Fibrous Glass
• Dimensions 14cmx17cmx20cm
• Flexible
• pH neutral
• Service temperature range -40C to 38C
• Non-combustible

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Design Details

• Voltage Temperature Sensor
• Typical Accuracy 0.5C
• Temperature Reading Range -40C to 125C
• Space Saving Package

11
Design Visual
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Control Flow Chart
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Calculations

• Control Volume 0.00476 m3
• Diameter of inlet/outlet tube 2 cm
• Maximal Flow Rate 0.228 L/s
• Heat Transfer Coefficient 2.9 W/m2K
• Required Heat Removal 2.76 W

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Weight of Components

• Vortex tube 500 g
• Fibrous glass 90 g
• Silica Gel 75 g (dry), 125g (saturated)
• Mounting Clip 15 g
• Connecting Pipe 250 g
• Velcro 2 g
• Thermistor 1 g
• Wiring/Circuitry 40g
• Total 1000g (1kg)

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Safety Analysis

• Users Operator, Technician, Electrician,
  Trainee, Non-user
• Tasks Normal operation, Maintenance,
  Troubleshooting, Inspection
• Hazards Fatigue, Radiant heat, Inadequate
  ventilation, Damage to machine, Live fault wires
• Low Risk
• Risk Minimization Training, Organized workspace,
  Signs warnings

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Parts Prices
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Conclusions

• Concerns
• Patent design
• No formal FDA approval necessary
• Accomplishments
• Simple practical design
• Met all specifications deadlines
• Future Direction
• Build a prototype
• Testing optimization

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5 Lessons We Learned During Our Project

• Engineering in the real world is HARD
• Teamwork communication are essential
• Compromises have to be made
• Commercial salesmen are annoying
• Google rocks

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Questions?
http//www.bmefosho.wo.to bmefosho_at_gmail.com