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Micropropagation of orchids disinfestation and plating of a shoot tip formation of a protocorm multiple shoots develop from protocorms shoots are separated, ... – PowerPoint PPT presentation

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1
Other specialized structures
  • tubers
  • tuberous roots
  • rhizomes
  • pseudobulbs

2
Propagation of Irish potato (Solanum tuberosum)
tubers
  • conventional method tuber is cut into sections,
    with an eye or node included tubers used for
    propagation are called seed potatoes
  • micropropagation veg. buds are excised, grown,
    multiplied in culture, handled to produce
    microtubers for virus-indexed seed stock
  • potato tubers are modified stems

3
Fig. 15-15 and 15-16. Propagation of Irish
potatoes by tuber pieces.
4
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5
Propagation by tuberous roots
  • Sweet potato (Ipomoea batatas)
  • adventitious shoots develop on the fleshy root
  • new slips are covered with soil, develop
    adventitious roots
  • Dahlia
  • plants are dug in the fall, divided
  • ea. divided section contains a tuberous root and
    a piece of the crown with a shoot bud

6
Fig. 15-18. Propagation of sweetpotato by
adventitious shoots from tuberous roots.
7
Fig. 15-19. Propagation of dahlia by tuberous
roots.
8
Figure of dahlia tuberous root division, showing
the right way and the wrong way (Free 1957)
9
Rhizomes
  • Defn specialized stem with the main axis of the
    plant growing horizontally at or below the ground
    surface
  • Types
  • Pachymorph a short, thick, fleshy clump,
    determinate (terminating in a flowering shoot),
    e.g., German iris
  • Leptomorph a slender stem with long internodes,
    indeterminate (growing continuously from the
    terminal apex) e.g., lily-of-the-valley)

10
Fig. 15-20 and 15-22. Photo and figure showing
pachymorph and leptomorph rhizomes
11
Division of rhizomes
  • pachymorphs rhizome sections are cut off,
    transplanted
  • leptomorphs - lateral offshoots (1st or 2nd yr)
    or pips (3rd-yr shoots) removed and transplanted
  • culm cuttings - culm (aerial flowering shoot) is
    laid horizontally, branches arise at the nodes
    (e.g., bamboo)

12
Pseudobulbs
  • Defn specialized storage structure of epiphytic
    orchids
  • Propagation methods
  • offshoots develop at the nodes of a long, jointed
    pseudobulb (e.g., Dendrobium)
  • rhizome division (Cattleya), cut back from the
    terminal end to include 4-5 pseudobulbs in each
    section
  • micropropagation

13
Fig. 15-24. Cattleya orchid rhizome with several
attached pseudobulbs.
14
Micropropagation of orchids
  • disinfestation and plating of a shoot tip
  • formation of a protocorm
  • multiple shoots develop from protocorms
  • shoots are separated, rooted, transplanted to soil

15
Fig. 18-11 and figure from Bhojwani (1983).
Steps in the micropropagation of orchids.
16
Recap
  • Tubers and tuberous roots
  • Rhizomes - types and propagation methods
  • Pseudobulbs and protocorms - propagation methods
    for orchids
  • And, from the text (Ch. 15) Who discovered that
    orchids could be vegetatively propagated by
    protocorms?
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