??????Wolbachia ? ?????

1
??????Wolbachia ??????

• ? ? ?

2

• ?????(Wolbachia)????????????????????????????16???
  ??????(Werren,1997)??????????????????,????????????
  ?????(feminization)??????????(parthenogenesis),???
  ??????????(Cytoplasmic Incompatibility,CI)
• ??Wolbachia?????????????????,??????????????????

3

• Wolbachia?????
• 1924?Hertig?Wolbach?????Culex pipiens?????????????
  ??,??????Wolbachia pipientis
• 1950?,Ghelelovitch?Laven???????????????,??????????
  ???????,Laven??????????????????(????),????????????
  ????(Cytoplasmic Incompatibility,CI)

4

• 1970??,?Yen ?Barr (1971)?????????Wolbachia?,??????
  ?????????????????????????,???????(?????),????CI???
  ?????????25??,??????????CI??,????????????????????
  ????????????
• ????CI???????????????,????????????????????????????
  ????????????????,??????????(?30???????)???????

5

• 1987?Legrand??????????????????????????(feminizatio
  n)?????????1990?Stouthamer????????????????????????
  ?,??????????????????
• ???ftsZ(??????)?16S rDNA?Wsp (??????)??????????PCR
  ,??????????????????( Parthenogensis Induction,
  PI)??????????????????????----Wolbachia

6

• Wolbachia???????????????
• ??,??Wolbachia????????????,?????????????,?????????
  ?????????
• ???Wolbachia???????????????????????????
• ?????????,Wolbachia???????????,?????????????????
  ????????,?????????????Wolbachia?????????????,?????
  ?????????????????????,???????

7
? Wolbachia????????????
???? ???? ???? ???
??????? 5 18 28
?????? 0 17 0
??? 5 8 62.5
?? 11 20 55
Aylacini 4 8 50
???? 3 52 5.8
????? 7 16 43.8
?????? 3 7 42.8
? ? 38 146 26.0
8
Wolbachia??????(PI)???

• ????Aphelinidae
• Aphytis chilensis
• Aphytis chrysomphae
• Aphytis diaspidis
• Aphytis lingnanensis
• Encarsia formosa
• ????Encyrtidae
• Apoanagyrus diversicornis ?
• ????Pteromalidae
• Muscidifurax uniraptor
• ???? Trichogrammatidae
• Trichogramma brevicapillum
• Trichogramma chilonis
• Trichogramma cordubensis
• Trichogramma deion
• Trichogramma nr.deion

9

• ???? Trichogrammatidae
• Trichogramma embryophagum
• T. evanescens
• T. kaykai
• T. nubilale
• T. oleae
• T. platneri
• T. pretiosum
• T. nr. Pretiosum
• T. siberocum
• T. nr. sivercum

10

• ???Cynipidae
• Diplolepis rosae
• Diplolepis spinosissimae ?
• ?????Eucoilidae
• Leptopilina australis
• Leptopilina clavipes

11
Wolbachia ???????(CI)???

• ????Pteromalidae
• Nasonia giraulti
• N. longicornis
• N. vitripennis
• ????? Eucoilidae
• Leptopilina heterotoma
• ??? Braconidae
• Asobara tabida
• ??? Ichneumonidae
• Diadegma chrysostictos
• Venturia canescens
• ??? Proctotrupoidae
• Trichopria drosophilae

12

• ??Wolbachia?????(CI)?????????????CI??????????????
  ??????Wolbachia????????????????,????????,?????????
  ?????????????????????????????Wolbachia???,???????
  ??????????????????

13
? ?????????????? (???,2002)
? Wol ? Wol F1????
?? ?? 63
?? 59
?? 71
?? 8
?? - 37
?? ?? 17
?? 78
?? 47
?? - 29
14
?? ?????????????? (???,2002)
? Wol ? Wol F1????
?? ?? 59
?? 29
?? - 39
?? - 51
?? ?? 15
?? 21
?? ?? 61
15
?? ?????????????? (???,2002)
? Wol ? Wol F1????
?? - ?? 0
?? 0
?? - ?? - 4
?? 0
?? 0
?? 0
?? 0
?? - 23
16
??????(parthenogenesis inducing)

• ??????,?????????????????????????(meiotic
  drive)??????????????????(cytoplasmic sex ratio
  distorers)?????????????????????????????,?????Wolba
  chia,??????????????????????????????,??????,???????
  ???????????????,?????????????????????????????????
  ?????????

17
???(feminization)

• ??????Wolbachia???????????????????????(Armadillidi
  um vulgare)??????????,??????????(feminizing
  parasitic sex factors,PSF)??,?????PSF?Wolbachia??(
  ???F)????????(??f),???????????F-
  Wolbachia?DNA??????????????PSF,???????????????(zz)
  ,???PSF?????(override)Z????????????,???????(??????
  ????zzF?zzf)?

18
Wolbachiar ?????????

• 1988?Stackebrand???????????Wolbachia
  pipientis????????, W. pipientis???????a-??(?)?????
  ???????????????????????????????? W.
  pipientis??????Anaplasma marginale?Ehrlichia
  risticiit ??????,??Anaplasma marginale????????????
  ?????, Wolbachia???????? Wolbachia persica????-???

19
(No Transcript)
20

• ???33???Wolbachia???ftsZ????,??????????,????A???B?
  ????????????,A??B ??58006700????????,????????????
  ??????16SrDNA????2?
• Wolbachia?????????(horizontal transmission)?????(v
  ertical transmission)???????B??????,??????????????
  ????????

21
Wolbachia?????

• 1994??,????S. Hoshizaki ????????????????9?????,???
  ??(Odawara),????(Furukawa)?????????????(??)???Wol
  bachia??,???????5???????,??????????

22
? ?????????
?? ??? ??(ºN) ????
FRK ?? 38.33 10 Oct 1994
SDI ?? 38.18 10 Oct 1994
KRY ?? 37.12 21 Sep 1994
YIT ?? 36.48 18 Sep 1994
UCH ?? 36.12 16 Sep 1994
TKS ?? 36.17 9 Sep 1994
KNS ?? 36.02 9 Sep 1994
URW ?? 35.53 4 Sep 1994
ODW ??? 35.17 2 Sep 1994
23
? ???9???????Wolbachia???
?? ????? ????? ????? ????? ???? ????
?? N N N N N P()
FRK 9 0 13 0 22 0
SDI 7 0 6 1 13 8
KRY 3 1 15 5 18 33
YIT 7 6 23 22 30 93
UCH 5 5 5 5 10 100
TKS 10 10 15 15 25 100
KNS 8 8 20 20 28 100
URW - - 3 3 3 100
ODW 4 4 17 17 21 100
24

• ??1984?Noda??????????,???????(???)????Wolbachia,??
  1994?Hoshizaki???,???????,??????????4?(???????????
  ????),??????????????????93?33?8,????????????Wol
  bachia????????????,??????????????????
  Wolbachia???????????????????
• ?????????Drosophila simulans???Wolbachia?????,3???
  ?????,?????100km??????

25

• ??
• ??Wolbachia???CI?PI???????,?????????????????
  ???????????????????
• Wolbachia??CI?????????,???????????,?????????
  ?????
• ??????????????????????,????????,??CI??,??????
  ????????,????????,??????????????,?????????(???)???
  ?

26

• ??Wolbachia?IP??,??Wolbachia??????????????????,???
  ???,?????????????????,??????????????
• ??????,?????????????????????,??????????????????
• ?????Wolbachia?????,???????,???????????

27
??????????(???)
??
????
?
???
??
??
?????
????
?
??
????
????
????
???
????
28
(No Transcript)
29
(No Transcript)
30
????

• ??? ??? ??? ??? ???.?????Wolbachia?????.????,2002,
  22(3)409-419
• ??? ??? ??? ??? ???. ??????????????????.????,2002,
  45(1)14-17
• ?? ??? ???. Wolbachia?????????????????????.????,20
  02,45(2)241-252
• Hertig M. The rickettsia, Wolbachia pipientis
  (gen. et sp. n.) and associated inclusions of the
  mosquite Culex pipiens. Parasitology,
  1936,28453-486
• Laven H. Speciation by cytoplasmic isolation im
  the Culex pipiens complex. Cold Spring Harbor
  Symp. Quant. Biol.,1959,24166-173
• Yen J H and Barr A.R. New hypothesis of causa of
  cytoplasmic incompatibility in Culex pipiens.
  Nature, 1971,232527-528
• Bourtzis K.dobson S.L. Braig H. R. et al.,
  Rescuing Wolbachia have been overlooked letter.
  Nature, 391852-853

31

• Werren J. H. Biology of Wolbachia. Annu. Rev.
  Entomol., 1997,42587-609
• ??? ??? ???. ??????Wolbachia??????????.
  ????(?????),1997,36(5)500-506
• ??? ?? ??? ?? ???. ???Wolbachia????????????.????(?
  ????),1998,37(4)542-546
• ??? ??? ?? ??? ???. Wolbachia?Drosophila
  auraria????Drosophila sinulans????.????(?????),199
  9,38(4)422-427
• ??? ??? ??? ??? ???. Wolbachia??????????????.????(
  ?????),2000, 39(3) 331-337
• ?? ?? ??? ?? ???. Wolbachia?????????.????(?????),2
  001,40(5)539-543

32

• ??? ??? ??? ??? ???. Wolbachia?wsp????????????????
  PCR??. ??????????, 2000,7(2)96-98
• ?? ???. PCR?????????????????????. ??,
  2002,24(2)207-210
• ??? ??? ??? ???. ?????????????????Wolbachia?????.
  ????, 2002, 45(6)705-710
• ??? ??? ??? ??? ?? ??. ?????????Wolbachia 16S
  rRNA???????. ???????, 2002,23(2)81-83
• ?? ?? ?? ??? ??? ???. ?????Wolbachia??????????????
  ???????. ????, 2002,47(2)916-921

33

• ?? ??? ???. ?????????????(Wolbachia)???.????,
  2002,39(3)188-190
• ??? ??? ??? ??? ???. ??????? Wolbachia
  ?wsp?????????. ????, 2002, 45(5)571-577
• ?? ??? ???. Wolbachia?????????????????????. ????,
  2002, 45(2)241-252
• ??? ??? ??? ??? ???. Wolbachia????????????????????
  ?PCR??. ??????????, 2002, 9(1)26-32

34
? ?
Thanks for your attention !