Rapid detection of sperm : comparison of two methods

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Rapid detection of sperm comparison of two
methods

• Miss Wimolmas Phorassamee 51312344

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Introduction
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comparison of two methods

• Acid phosphatase test
• Zinc test

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Component of sperm
Acid
Spermatozoa
Enzyme (Protease, phosphatase)
Semen
Protein
Seminal plasma
Enzyme
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Method
174 cervicovaginal samples from consenting women
Cytology
Acid phosphatase test
Zinc test
Statistical
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Result
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Result (con.)
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Result (con.)

• Acid phosphatase
• - sensitivity 95
• - specificity 96
• - PPV 93
• - NPV 98

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Result (con.)

• Zinc test
• - sensitivity 81
• - specificity 54
• - PPV 49
• - NPV 84

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Discussion Conclusion

• the acid phosphatase test has good sensitivity
  and specificity, and above all that it has a
  negative predictive value of 98
• It is thus valuable as a screening test
• the longest timespan after which detection was
  possible was 106 h between intercourse and
  sampling

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Discussion Conclusion
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Thank you