Biofilms, Methylation

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Biofilms, Methylation Heavy Metal
Detoxification in Lyme Disease

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Overview

• Biofilms
• Gastrointestinal physiology
• Definition of Biofilms
• Examples
• Prevalence
• Treatment
• B. Heavy Metals
• Prevalence
• Signs symptoms
• Testing
• Treatment options
• Methylation in non-responders
• C. Conclusion

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B. Biofilms in Lyme Disease 1. GI
Physiology Structure of normal intestinal
lining

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B. Biofilms in Lyme Disease 1. GI
Physiology Structure of intestinal lining in
Gluten intolerance

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B. Biofilms in Lyme Disease 1. GI
Physiology Microbial Flora
I. One hundred trillion bacteria in
gut comprises 500 different species
II. Disruption early leads to immune problems,
allergies, and autoimmunity later
III. Functions modulates immune system
destroys toxins introduced with food
suppress growth of pathogenic bacteria
production of key vitamins digestion
and absorption of carbohydrates
prevention of allergies prevention of
inflammatory bowel disease

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B. Biofilms in Lyme Disease 2. Definition
a. Community of bacteria and
other organisms surrounded by a extracellular
polymeric substance (EPS) b. EPS is composed
of DNA, protein, and polysaccharides. Its
negative charge attracts Ca/Mg/Fe to strengthen
it c. Organisms within a biofilm can
communicate and exchange genetic material.
d. EPS provides resistance to antibiotics
requiring 100-1000X higher levels for
eradication. Testing the
susceptibility of bacteria in biofilms to
antimicrobial agents . Antimicrobial Agents and
Chemo. Nov 1990 e. EPS provides this
organisms protection from UV exposure, pH
changes, heavy metal toxicity, and phagocytosis.



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B. Biofilms in Lyme Disease 3. Examples of
Biofilms a. Teeth b.
Catheters and IV Lines c. Stagnant pools of
water, rivers and streams d. Contact lens e.
Polluted areas f. Blood (Fry et al)

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B. Biofilms in Lyme Disease 4. Prevalence
of Biofilms a. Autism Spectrum
Disorders b. Chronic Lyme Disease c. Chronic
Fatigue Immune Dysfunction Syndrome d.
Fibromyalgia e. Autoimmune Illness

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B. Biofilms in Lyme Disease 3. Examples of
Biofilms

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• B. Biofilms in Lyme Disease
• 5. Role of EDTA in Dissolving Biofilm
• a. EDTA serves to bind and remove the Ca/Mg/Fe
  holding biofilms together
• b. Staph biofilms could not be eradicated by
  Vancomycin or EDTA alone. Together the two
  agents successfully removed the biofilm (Kim
  2005)
• c. EDTA and Gentamycin are 1000X more effective
  at killing Pseudomonas than either agent alone.
  This effect is completely blocked when Ca or Fe
  are added (Banin 2005)

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• B. Biofilms in Lyme Disease
• 6. Treatment of Biofilms
• a. Enzymes
• b. EDTA
• c. Antimicrobials
• Antifungals
• d. Fiber
• Brown Algae
• Modified Citrus Pectin
• Activated Charcoal
• Zeolite
• e. Probiotics
• Prebiotics (fresh fruit, legumes, chicory,
  FOS, inulin)