Ginsenoside Phytochemical Variation

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Ginsenoside Phytochemical Variation

• Kristina McIntyre
• University of Ottawa

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Objectives

• Examine the variation in ginsenoside composition
  between 5 Ontario ginseng farms
• Inter- and intra-population variation
• 6 main ginsenosides Rg1, Re, Rb1, Rc, Rb2, and
  Rd
• Broader range
• Identification of unique ginsenoside profiles and
  landraces

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Preliminary Work

• Chromatographic methods of analysis
• Examination of Variation in 1 population
• Examination of ginsenoside AGE-inhibition activity

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Recovery Study
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Ginsenoside Variation- Population 1
Figure 1. HPLC-DAD chromatogram of the 6 major
ginsenosides in Panax quinquefolius.
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Ginsenoside Variation- Population 1
Figure 2. Relative abundance of 6 ginsenosides
and total ginsenosides (w/w) averaged across
Panax quinquefolius roots on one Ontario farm.
Mean values (n17) standard deviation are
presented.
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Ginsenoside Variation- Population 1

• Total ginsenoside content ranged from 4.9 to
  13.7 (w/w)
• Mean of 8.4(w/w)
• Greater than the total ginsenoside in New York
  State, Maryland, British Columbia, wild
  populations in Ontario, Quebec, Vermont, Maine,
  and Wisconsin which range from 1.53- 5.78
  (w/w)
• Rb1, Re, Rd dominant
• Rg1 and Rb2 low quantities

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AGE Inhibition Assay

• Experimental
• BSA glucose/fructose extract
• Controls
• Positive, Negative, Extract, BSA, Vehicle
• Quadruplicate to 96 well plate
• Incubate 7 days, 37oC
• Read fluorescence- 355 and 460nm
• Determine percent inhibition and IC50
• Inhibition
• ((Fnegative- Fexperimental corrected)/ Fnegative
  )100

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AGE-inhibition
Table 2. Summary of data obtained for the IC50
ranges of major ginsenosides and Naturexs
ginsenoside extracts
Ginsenoside IC50 Range (mg/mL)
Rb1 1 - 10
Rd 1 - 10
Re gt 10
Rg1 0.5 - 1
Naturex aqueous extract gt 10
Naturex hydro-ethanolic extract gt 10
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Conclusions and Future Work

• Significant intra-population variation
• Low AGE-inhibition activity
• Examine inter-population variation
• Examination of a wider range of ginsenosides
  (including malonyl ginsenosides) and phenolics
• Further study of biological activities

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Questions or Comments?