2nd Induction

1
Supplementary Figure S2
2nd Induction 1mM H2O2
Only H2O2 concentrations capable of inducing the
GSTF8 promoter can activate desensitization
Plates containing three rows of four-day
GSTF8LUC seedlings were pretreated with
different concentrations of H2O2 14 h prior to
1mM H2O2. For each treatment, 3mL was added to a
55 mm MS plate containing 50mM luciferin and
decanted after 40 min. The bioluminescence was
measured for each seedling 6 h after after 1st
and 2nd treatment using a CCD camera. The purple
images reflect the fluorescence of the
chlorophyll, while the green represents the
luminescence expression from the active
GSTF8LUC construct.