1.?????: ???????????? ??????(??Folin-Wu?) ???? ??? ??????(Cu) CuO ??? ??

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?.??????????(?)???????????
?????????,???????????
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1.????? ??????????????????(??Folin-Wu?)????
??? ??????(Cu) CuO ??? ??
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?????? ????????,??? ???? ????
????? ?? VitC ????
??
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2.?????? ???????????????? Hultmen????????(Otolu
idine,O-T)????????? ?? ??? ????
(???) 5???2???? ????
??????(Schiff?)
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3.????? ?????????????(Glucose
Oxidase,GOD)???? ????????? ????
????H2O2 4-?????? ? O2 ?????
??????????????
?????
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????????? ????,????? ????
??(???gt0.34mol/L) VitC 5mg/L??
????(0.5mol/L) ???? 100mg/L??????????,??
?????,??????????????
???
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??? ATP G6P ADP G6P NADP
6-?????? NADPH NADPH??????????????,???340nm?
????????,???????????  
??????????
????
G6PD
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???????? ???? ???? ????
?????? VitC NaF????EDTA????
???????????????????????NADP,???? ????????
???
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(?) ??????? ????????? ???24???? lt 0.5g
???? ()1.????CuSO4 Glu Cu???2.?10A???????
???
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(?) ?????????(Oral glucose tolerance test ,
OGTT) ?????????,??????????????
??????67mmol/L,OGTT OGTT ????? a
?????,???????150g b ????????3d c
?????1016h??? d ????5min??250ml?75g???
???,????30min????,? ??,??2h?
11
mmol/L
10
???
???
?????
5
???
30 60 90 120 150 (min)
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1.?????(1)?????????lt6mmol/L(2)??????3060min???,
? ????10mmol/L(3)2?????????? 
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2.??????? ?????????????????,????????????
??????????gt7.8mmol/L ?????10mmol/L?????
???(2???)??????? ??????????2???????????? 
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3.????? ????????(1)???????lt7.8mmol/L(2)??????
30?60?90min??? ????11mmol/L(3)2h??811mmol/
L ????????????? 1/3???? 1/3???????
1/3?????
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 4??????????? ?? ?? ?? ?? ??
????? ???? ????????????????????????????