Hind III 400. Bam HI 2275. Bgl II 600. KanR. Extract the fragments for the gel ... Digest with Hind III and Bam HI. 3755. 2332. 1875. E. coli strains ...
... genes -lactamase can destroy penicillin and other -lactam antibiotics Kanamycin interferes with Ribosomes Plasmids can be cut with restriction enzymes ...
T4 Lig (ligase) - DW (distilled water) ... to 'Lig' tube for the ligation reaction. Make sure to add ... tube marked 'Lig' when you dispose of your other ...
Observations of Multiple Transformation Kits Bryan Smith (LaLumiere School) Teresa Pairitz (Marian High School) Shelly Gregory (South Bend Adams High School)
Pediatric Neurogenetics Zheng (Jane) Fan, MD Medical Genetics Fellow UNC-CH 04/2006 What is Neurogenetics? Neurogenetics: the study of genetic factors that contribute ...
iGEM Principle of parsimony occam s razor What is iGEM? iGEM stands for iNternational Genetically Engineered Machines An undergraduate bioengineering ...
Two DNA Size Standards. One Kilobase Ladder. A Synthetic Mixture of Molecules (3 kb brightest) ... Lambda DNA cut with the restriction enzyme (RE) HindIII ...
Each has its own unique cleavage sequence, which can appear several times in a DNA molecule ... Lower percentage: big pore size, good for separating large fragments ...
Hind III. Lambda. Result Brown. unkown. Result Green ... Hind III. Linear DNA. Result Restriction Digest & Gel electrophoresis. Lane 1 2 3 4 5 6 7 8 9 10 ...
Plasmid Digest. NOTE: The buffer is always ... Plasmid Digest. Place the reaction tubes in a 37oC water bath and incubate for 30-60 minutes. ... Plasmid Digest ...