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Cleavage-and-polyadenylation specificity factor (CPSF) binds via recognition of ... Nature Structural Biology 7:838, 2000 and Barnard D.C. et al, Cell 119:641, 2004. ...
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Finally, binding of poly(A)-binding protein II (PABII) binds to the initial ... Adapted from Textbook 'Molecular Cell Biology' Lodish H., Berk A., Zipursky L. ...
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RNA Degradation by the Exosome Is Promoted by a Nuclear Polyadenylation Complex ... Physiological condition (150mM salt) 500 mM salt ...
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... * Non-coding RNAs * RNA processing pre-RNA mature-RNA * Examples of RNA processing: 1. Capping of 5' end. 2. Polyadenylation of 3' end. 3.
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... Intron INTRON 1st EXON Mid EXON Last EXON 1.5kb 100nt 800nt 5kb 150nt 800nt 150nt Short Polyadenylation signals RNA Editing MicroRNAs Northern RNA blot ...
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CHAPTER 15. Messenger RNA Processing ?. Capping and Polyadenylation. fan lou kong (???) ... Functions of Ploy(A) 1.Protection of mRNA. The Figure 15.10 examined it. ...
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Capping - Splicing: cis, trans, & alternative - Polyadenylation ... Post-transcriptional Steps of Gene Regulation. R-Loop Experiment Reveals Introns ...
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Why study RNA? Gene expression regulation post-transcriptional regulation: polyadenylation splicing editing transport translation stability - RNA molecules can ...
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In the previous two chapters, we examined splicing, capping, and polyadenylation. ... a 100-nt fragment will appear to corroborate the trans-splicing hypothesis. ...
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... greater than 91% homology between chick brain, mouse brain, and human ... A single polyadenylation site in the chick clone was also present and duplicated ...
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0 3 6 10 15 20 30 40 50 60. Tell me in detail how this experiment ... deleterious effect. Premature Polyadenylation Occurs. Frequently at Many Different Sites ...
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cataloging system to organize a library. a library of multi-media objects ... Telic: RNA polymerase has-purpose to be a Catalyst in Polyadenylation. Imagery: ...
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1. Capping at the 5'-end. 2. Polyadenylation at the 3'-end ... The cap serves several functions, including protection of the mRNA from ...
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Digest the labled capping substance(xp) to yield x by phosphomonoesterase,then ... II leave the complex; PAP enters .aided by CPSF initiates short poly(A) synesis. ...
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Cap 1 found in both eukaryotic and viral RNAs. Cap 2 found only in eukaryotic cells ... More variation exists in plant than in animal motif ...
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A complex network of coupled interactions in gene expression ... RNA editing creates a. Stop codon. More RNA Editing. Trypanosome. Pan Edited. COIII mRNA ...
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Regulating gene expression Goal is controlling Proteins How many? Where? How active? 8 levels (two not shown are mRNA localization & prot degradation)
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An efficient site-specific transposition system to generate baculovirus for high ... pFastBac donor plasmid vector into which the gene(s) of interest will be cloned ...
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Title: Introduction to Virology Author: Mike Last modified by: Created Date: 11/27/2006 4:44:40 PM Document presentation format:
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Transcription initiation: The TATA box. RNA Polymerase. Assembly: Transcription Initiation ... Theory regarding the evolutionary origin of introns: ...
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Protects mRNA from ribonucleases: mRNA's without caps rapidly ... MT binds to CE (mammals) or P-CTD (yeast) and to the terminal GpppN (mammals). c, Imp ...
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... transcribed by RNA Pol II ... cleavage factor II PAP: Poly(A) polymerase CPSF PAP PABPII- poly(A) ... different from mRNA p p Pol II ctd mRNA SCAFs: ...
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Nucleolus. For rRNA processing. Nucleolus and other subcompartments ... Nucleolus with sub-compartments. From RNA to Protein. Protein synthesis ...
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Post-transcriptional RNA Modifications Gene Expression Part 2 Post-transcriptional Modifications Processing cleavage of 45S rRNA precursor, base modifications of ...
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Covers : RNA Processing Translation Genetic Engineering Membrane Transport
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SXL and TRA are SR RNA-binding proteins (ser arg rich) DSX is a class II transcription factor ... Exons can contain signals recognized by SR proteins ...
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Activation of gene structure. Initiation of transcription. Processing transcript ... Stability of Eukaryotic mRNA. Both the 5' and 3' ends of mRNA are modifed ...
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Gene targeting Maximizing the targeting frequency and selection of targeted clones Random integration predominates --- design vector to increase the targeted ...
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Mechanisms of Transcription RNA polymerase does not need a primer to initiate transcription. The RNA product does not remain base-paired to the template DNA strand.
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Title: PowerPoint Presentation Author: Shai Last modified by: Shai Created Date: 1/1/1601 12:00:00 AM Document presentation format: On-screen Show (4:3)
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Base pairing of U2 snRNA with the branch point is thought to displace and activate the adenosine whose 2 OH attacks the 5 splice site * * snRNP snRNA length ...
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How cells read the genome: from DNA to protein
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Controlling the rate at which the mRNA decays can regulate the levels of ... (dsRNA) introduced into the nematode worm, C. elegans, and is largely conserved ...
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Enhancer. Any combination of transcription factor binding sites ... Enhancer. Tissue specific txn factor and cognizant DNA element (site) Epigenetic Gene Regulation ...
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a more accurate outline of the events involved in genome expression, especially ... duquel se trouvent deux ions Mg 2 qui indiquent l 'emplacement du site actif. ...
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ANTIBODY STRUCTURE AND THE GENERATION OF B-CELL DIVERSITY WHAT ARE ANTIBODIES? Antigen specific proteins produced by plasma cells Belong to immunoglobulin superfamily ...
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Transcription by RNA Pol I an II. Regulation of transcription in eukaryotes: ... Introns (non coding sequences) are cut out from the pre-mRNA. ...
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Bioinformatic analysis of the beta-secretases, BACE1 and BACE2: integrating ... The Genomic Neighbourhood of Zebra fish (Danio) BACE1 & 2. BACE1. T-box. Apo A. BACE2 ...
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Use of abnormal polyA site is associated to various diseases: A/B Thalassemia (globin) ... Consistent with recent tiling microarray data (Rosetta etc. ...
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Semi-packed DNA: One Chromosome. DNA Packing. RNA Structure ... Can be double-stranded (viral genome), no helix. Can form DNA-RNA hybrid helices ...
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Immunoglobulins Generation of Diversity
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Chap. 8 Post-transcriptional Gene Control Topics Processing of Eukaryotic Pre-mRNA Regulation of Pre-mRNA Processing Cytoplasmic Mechanisms of Post-transcriptional ...
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Probe selection for Microarrays Considerations and pitfalls
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Overview of methods for plant transformation ... produces filaments resembling the pili used for interbacterial conjugation.(VirB) ...
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Major RNA processing mechanisms in eukaryotes. ribosomal RNA ... Tissue-specific processing of the calcitonin primary transcript. Nelson & Cox, 2005, p. 1015 ...
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'Dependence on another organism that does harm to the host. ... J.E. Taylor, G. Rudenko, Switching trypanosome coats: what's in the wardrobe. Trends Genet. ...
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Gene Structure and Identification Eukaryotic Genes and Genomes Gene Finding Previous reading: 1.3, 9.1-9.6 Reading: 10.2, 10.4, 10.6-8 BIO520 Bioinformatics Jim Lund
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... retained portion of intron 1 results in termination at a stop codon in intron 1 ... The GAG glutamate codon is mutated to an AAG lysine codon in Hb E ...
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A single V can rearrange to more than one C. Multigene families. or. In humans: 40 V , 5 J , 1 C ... Introduction of cloned genes into mice (and other animals) ...
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On average, a vertebrate gene is about 30KB long. Coding region takes about 1KB ... Esngl single exon (intronless) (translation start - stop codon) ...
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A. Control of gene expression by binding of trans-acting protein ... 2. The helix-loop-helix motif. 3. The helix-turn-helix motif. 4. The zinc finger motif ...
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4.The RNA product does not remain base-paired to the template DNA strand. (Figure12-1) ... Only one of the DNA stands acts as a template. ...
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