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... instruct a multicomponent nuclease, RISC, to destroy specific messenger RNAs. ... The RISC. complex. SiRNA and miRNA. MicroRNAs. Genomics, Biogenesis, ...
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... the transition of the Hammerhead ribozyme to its catalytic conformation Journal of Biological Chemistry 272 (1997) 26822-6.
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(RNA Enzymes) David Peterson Enzymes Catalyze reactions Very specific Act on substrates Active site Enzymes Bind Substrates Enzymes Bind Substrates Enzymes Bind ...
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Before the discovery of ribozymes, only proteins were known to have catalytic activity. ... These results lent credence to the possibility that splicing activity was ...
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... B enables the conserved regions to approach each other, buries the active core. ... 5 -OH leaving group buried deeply between two domains ...
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25 Years of Ribozymes
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RNA strand scission. RNA splicing. Peptide bond ... RNA strand scission ... and ribozymes catalyze phosphodiester bond scission by a variety of mechanisms ...
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Mechanisms of Apoptosis/Review Ribozyme Inhibition of Bcl-2 Expression/Summary Sandra A. Gibson Hudson Ph.D. APOPTOSIS Morphology Purposes of programmed cell death ...
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Scissile phosphate is labelled with an asterisk. ... Conformational changes after scissile bond breakage discharge the catalytic ...
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Here, using small-angle X-ray scattering, the authors show that Mg2 -induced ... tertiary contacts between the L9 tetra-loop and its helical receptor ...
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A catalytic active RNA fold will ligate the nicked DNA which has a biotin tag. ... This adenylylation mimics the first step of the ligation mechanism of T4 DNA ligase. ...
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Supplementary Figure 1 Sequence and cleavage mechanism of ncRNA ... sequences through unique restriction sites BamHI, EcoRI, SalI, and XhoI (indicated in green) ...
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... (DNAzyme) RNA RNA (ribozyme) ...
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... 2006 HDV RIBOZYME The only catalytic cleaving RNA ... domains bipartite nuclear localization signal activation of reporter genes arginine-rich motifs ...
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???????????????????????? ?????????????? (Ribozyme or Catalytic RNA) ... ????????????? Hydrolysis. Esterases. Proteases. Phosphatases. Amylases. Peptidases. Ureases ...
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Catalytic Nucleic Acids: Ribozymes and Dnazymes. Triplex ... Pharmacology, Vol. 131, 'Antisense Research and Application', Ed.Cooke, S.T. ...
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DNA later took over the genetic role and proteins took over the ... The RNA world arose de novo in the form of self replicating ribozymes. Almost certainly true ...
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GAL1 / ribozyme construct expressing theophylline aptamer. pSWITCH1.aptamer. pRS316-Gal ... GAL1 / ribozyme construct expressing s1 and s9. pSWITCH2.s1/9. pRS316-Gal ...
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Gene Therapy antisense oligonucleotides(ODNs) Ribozymes DNAzymes RNA interference(RNAi). Several approaches for correcting faulty genes A normal gene may be inserted ...
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Self-splicing uses the intron in a stoichiometric fashion. ... Active sites in Group I intron self-splicing. Domains of the Group I intron ribozyme ...
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Hammerhead Ribozymes. Mechanism of Action of Antisense Oligonucleotides. RNA Interference (RNAi) ... therapeutic tool is aimed to treat the SARS and bird Flu ...
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RNA switches can be used in gene therapy, allowing the patient to take pills to ... constructed using seven distinct effector-modulated ribozymes based on the ...
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Figure 28.31 of Mathews. Secondary structure model of human ... RNA enzyme = Ribozyme' (look at pages 395-397 in Mathews) This type of splicing requires only: ...
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Non-coding RNA has been discovered to play an ever more important role in all ... whereby the VS ribozyme kinetically selects the distal downstream substrate ...
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Molecular biology basic- DNA, RNA structure, chargaff rule, nucleic acids, ATP, watson and crick model, types of DNA- A DNA, B DNA. Z DNA, types og rna
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Functional RNA Introduction
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Title: Author: xuyuquan Last modified by: Created Date: 10/4/2002 8:40:51 AM Document presentation format:
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Molecular beacons as probes of RNA unfolding under native conditions ... mM Mg2 , before unfolding was triggered by the addition of EDTA (data not shown) ...
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Operator. Open reading frame. mRNA. Bacteria Must Regulate Gene Expression ... Synthesis of glucosamine-6-phosphate is essential for survival in many bacteria ...
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LEQ: How are information-storing biomolecules proposed to have evolved? Reading: 12.3 (finish); 3.1, 3.3; test monday Activator: enzymes Biological catalysts are ...
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In principle, every step required for the process of gene expression could ... Grate D, Wilson C.Inducible regulation of the S. cerevisiae cell cycle mediated ...
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Nucleic acid aptamers Aptamers: molecules that bind other molecules with good affinity and specificity Usually these are proteins . . . . But they can also be RNA or DNA.
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Kinetics and Thermodynamics Make Different. Contributions to RNA Folding In Vitro ... Elisabeth M. Mahen, Jason W. Harger, Elise M. Calderon, and Martha J. Fedor ...
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In Vitro Transcription of 26S rRNA Gene Yields Excised Intron ... Lends credence to models in which a late stage RNA world had diverse metabolism and pathways ...
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University of Minnesota & Harvard Medical School ... moiety 'Tyrosine' moiety. P. P. messenger RNA. DNA. Puromycin. P. P. P. ribosome. mRNA-displayed protein ...
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Merriam Webster's Collegiate Dictionary. Helix (n) a. 'Something spiral in form. ... 10.5 bp/helix repeat. Helix diameter ~ 20 . 36 bp ...
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Results - Ligand recognition and pH dependence. Conclusions and future structural analyses ... Clare Boothe Luce Program of the Henry Luce Foundation ...
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Title: No Slide Title Author: Ross Hardison Last modified by: Ross Hardison Created Date: 10/21/1998 9:49:43 AM Document presentation format: On-screen Show
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Co-Evolution of the Genetic Code and Amino Acid BioSynthesis An hypothesis from 1975 by Jeffrey Tze-Fei Wong Anna Battenhouse
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Evolu n genomika Eduard Kejnovsk Kapitola II.: Relikty sv ta RNA
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www.biotech.kth.se. Skolan f r Bioteknologi. Biokemi (17) Nanobioteknologi (5) ... Lipase B from Candida antarctica is very active and stable in most organic solvents ...
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Chapter 2: Nucleic Acids, Genomics and Proteomics ZHOU Yong Department of Biology Xinjiang Medical University Teaching Requirements 1. Mastering: components and types ...
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Enzymes receive 'common' names reflecting their function, either in the forward ... Enzymes often require the participation of other small molecules to carry out a ...
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... ratio that is characteristic of molecules produced by ... all use ribosomes in the same way (translation) all ribosomes are composed of RNA protein ...
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( 3)????,???? (?????)(4)????????,?????????( 5)????????,??????????????? ... are clearly much more complex than nematode worms, yet only have about 50% more genes. ...
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Title: PowerPoint Presentation Author: Jeff Malen Last modified by: fcss Created Date: 5/20/2002 2:04:43 AM Document presentation format: On-screen Show
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The Big Picture How many processes can you name? http://www.youtube.com/watch?v=JY7nQ_5o_zo&feature=related
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Energy and Metabolism Chapter 6 Flow of Energy Energy: the capacity to do work -kinetic energy: the energy of motion -potential energy: stored energy Energy can take ...
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Nextgeneration DFTbased quantum models for simulations of biocatalysis
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Contribution of repetitive sequences to genome expansion Composition of human genome Repeated sequences comprise ~ 45% of total human genome !! Gregory Nature Rev ...
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telomerase RNA (telomerase maintenance) siRNA (RNA silencing) ... CRP/cAMP (68) MicF. 93. Anti-ompF. SoxR/S (22) MicC. 108. Anti-ompC. Inverse to MicF (19, 19a) ...
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Supernatant. Spin 2 hours at 78,000 x G. Spin 2.5 hours at 105,000 ... Supernatant (Cytoplasmic fraction) Pellet (nuclei, large membrane fragments, cell debris) ...
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The Hepatitis B Antivirals: Mechanism to Drug ... Virology 101: Understanding Hepatitis B at its Core ... Morrissey et al. Journal of Viral Hepatitis 2002. ...
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The History of Life on Earth AP Chapter 25
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