Unretained PowerPoint PPT Presentations

Chromatography. Physical separation method based on the differential ... Column Chromatography the stationary phase ... tm = retention time of unretained peak ...

Title: PowerPoint Author: Fleminger Last modified by: Gidi Created Date: 11/23/2002 4:37:37 PM Document presentation format:

Introduction to chromatography tecniques

It deals in detail concept of thin layer chromatography (TLC)

Peak integration by Dr.K.Balamurugan Group leader (R&D) INTRODUCTION Peak detection and integration are fundamental tasks in chromatography, most often done using ...

Peak integration by Dr.K.Balamurugan Group leader (R&D) INTRODUCTION Peak detection and integration are fundamental tasks in chromatography, most often done using ...

INSTRUMENTATION

Introduction Solvents & Reagent Quality Mobile phase preparation & usage Buffer, Filtration, Degassing Storage & usage Preparation for analysis Sample preparation & System suitability Resolution, peak symmetry, tailing, capacity factor, Adjustment allowed as per USP Column management Washing and change of mobile phase Analysis and integration Audit trail, data backup, Review and Documentation Problems and reporting Qualification and Calibration

V2 = volume of solvent 2 (extraction solvent) Extraction is a power function. 3 ... Gel Permeation. Similar to HPLC. 9. Immobilized Antibody Separation. 10 ...

Chem. 230 10/07 Lecture GC Instrumentation Sample Injection Gas Samples Fixed Loop Injectors A loop of fixed volume is filled with a gas The injection valve ...

1. Hydrophobicity (capacity factor of ethylbenzene) 2. Chelation (tailing factor of quinizarin) ... Column hydrophobicity (H) - increase in total carbon H increase ...

Eluent Strength. Strong mobile phase compositions make solute elute faster. ... polar mobile phases have higher eluent strength. Reversed Phase ...

... Extractions Quantitative calculations How to determine if method is working and how to improve methods Low Performance Chromatography Lower ... (Flash) means for ...

analyte and matrix must have differing affinities for one ... temperature (programmable) T. quality of column packing column type. The 'General Elution Problem' ...

Chem. 230 9/30 Lecture

Fast and Ultrafast HPLC on Sub-2-m Porous Particles Where Do We Go from Here? Column Watch Ronald E. Majors Column Watch Editor Higher productivity and faster ...

Introduction to Liquid Chromatography Columns System Components Applications Troubleshooting Susan M. Steinike, M.S HPLC Marketing Department ...

The chromatograph is the instrument, the chromatogram is the output! ... (depending on how much they like it) in the stationary phase in the chromatograph. ...

Chem. 230 9/11 Lecture Advanced Extraction Techniques SPE Demonstration Procedure: Clean cartridge with removing solvent, then sample solvent Apply sample ...

Mikhail Tswett invented chromatography in 1901 while researching on ... The inset shows that square root of resolution is. proportional to the number of passes. ...

1. Understand how R26 operates generally with respect to experts. ... A Doctor who operates on Bob is an 'expert,' under 26(a)(2)(A), but not, without ...

... just because facts are conveyed to an expert, they do not become undiscoverable: ... List of witnesses. Will call / may call. Depo degs. Exhibit list ...