Biochemistry320

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Biochemistry320 SectionB1 Winter
2007 MWF 1200-1250 Education North Rm 2-115
Lecturer Dr. Andrew MacMillan Questions/Appointm
ents andrew.macmillan_at_ualberta.ca Office Hours
by appointment, Medical Sciences 4-39 Course
Administration Rachel Milner
rachel.milner_at_biochem.ualberta.ca
Medical Sciences
579 Text Lehninger Principles of
Biochemistry (2005) by Nelson, Cox
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Date Lecture Chapter Jan. 8 No
Class Jan. 10 Introduction 1,2 Jan.
12 Amino Acids - 1 3 Jan. 15 Amino Acids -
2 3 Jan. 17 Protein Purification 3 Jan.
19 Primary Structure 4 Jan. 22 Secondary
Structure 4 Jan. 24 Tertiary
Structure 4 Jan. 26 Structure
Determination 4 Jan. 29 Protein
Folding 4 Jan. 31 Hemoglobin/Myoglobin 5
Feb. 2 Enzymes I 6 Feb. 5 Enzymes
II 6 Feb. 7 Mid-term I (lectures 1-12)
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Date Lecture Chapter Feb. 9 Chemical and
Enzyme Kinetics 6 Feb. 12 Catalysis Serine
Proteases 6 Feb. 14 Catalysis Acid
Proteases 6 Feb. 16 Review Feb.
19-23 READING WEEK N.B. March 19 Midterm
II (Lectures 12-24) Final Exam Cumulative
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Web CT http//www.ualberta.ca/ELEARNING
http//www.biochem.ualberta.ca/MacMillanLab Cour
se Notes
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Biochemistry
-identity and structures of bio-molecules
-interactions of bio-molecules with each other
-synthesis and degradation of bio-molecules
-energy creation, use, storage
-information storage, transmission, expression
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Ribosome X-ray Structure
Carbohydrate Structure
E. Fischer, Nobel Prize 1903
Science (2000) 289920-30
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Classes of Biomolecules
-peptides, proteins (co-enzymes)
-carbohydrates
-lipids
-nucleic acids
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B-Form DNA
Dickerson and coworkers (1980)
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It has not escaped our notice that the specific
pairing we have postulated immediately suggests
a possible copying mechanism for the genetic
material. From J.D. Watson and F.H.C Crick
Nature April 25, 1953
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Central Dogma
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Central Dogma
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Central Dogma
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Peptides/Proteins
myoglobin
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Carbohydrates
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Nucleosides and Nucleotides
ATP Hydrolysis
ATP
DG -7.3 kcal/mol
ADP Pi
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Lipids
phosphatidyl choline
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Biochemical Messengers
progesterone
NO
nitrous oxide
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Periodic Table
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Chemistry Review!
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Reactions
B
A
endothermic exothermic
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Energy
DG -RT ln Keq
DG DH - T DS
free energy, enthalpy, entropy
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Maximum disorder was our equilibrium

-T.E.Lawrence (Seven Pillars of Wisdom)
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Driving Forces in Biochemical Reactions
-concentration effects (Le Chatelier) -compartment
alization -irreversability -coupling
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Functional Groups
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Functional Groups
O
Carbonyl
O
O
H
R
O
Carboxylic Acid
Acyl
O
O
R
N
O
H
Amido
Ester
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Water
Key properties ionization, polar nature
m.p. 0C
b.p. 100C
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Henderson-Hasselbach
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Titration Curves of Acids and Bases
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Water
-polar solvent, can interact with both positive
and negative ions, other polar groups
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Hydrophilic and Hydrophobic Groups
Hydrophobic
Hydrophilic
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Hydrophobic Effect
Entropy and Structure of Bulk Water
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Interactions Within/Between Molecules
Covalent 60-100 kcal/mol
Hydrogen Bonding 1-5 kcal/mol
Van der Waals 0.2 - 1.5 kcal/mol
Ionic Interaction 1-10 kcal/mol