3Cpro wt CM IEALFQ?GPPKFR

1
GFP2
DsRed2
3Cpro wt CM IEALFQ?GPPKFR 3Cpro mut CM
IEKLFQ PPPKFR
Figure S1. Diagram depicting the FRET probe for
detecting the 3Cpro activity in vivo. Composition
and linear arrangement of the GFP2 and DsRed2
reporters are illustrated. The 3Cpro cleavage
motif (3Cpro wt CM) and the corresponding mutant
motif (3Cpro mut CM) in the linker are shown,
with the arrow indicating the scissile bond of
3Cpro and the underlined residues representing
the mutations in the P4 and P1 positions.
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