Prokaryotes

1
Prokaryotes

• Kingdom Monera or Prokaryotae
• two subgroups
• Eubacteria
• Archaeobacteria

2
Two subgroups

• Eubacteria typical heterotrophic bacteria,
• photosynthetic cyanobacteria
• pathogens
• Archaeobacteria
• halophiles
• thermophiles
• acidophiles

3
Bacteria

• binary fission, type of reproduction
• peptidoglycan, cell wall composition
• flagella composed of flagellin
• ribosomes, small and free in cytosol
• nucleiod for a circular dsDNA
• ubiquitous
• colony tens of thousands of organisms all
  originating from a single cell that underwent
  binary fission

4
Bacterial Morphology

• Three basic shapes
• coccus
• bacillus
• spirillum
• draw the three basic shapes

5
Bacterial Culture and Staining

• grown in
• broth
• agar (Petri plates or slants)
• Gram stain divides bacteria
• Gram positive
• Gram negative

6
Biochemical Identification

• biochemical tests for definitive identification
• Enterotube Multitest System, with
• 12 media
• 15 reactions