Lice Detection Test Presentation Prepared by: Paul Young

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Lice Detection TestPresentation Prepared
byPaul Young Narelle Sales

• Bovicola ovis The Sheep Biting Louse

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How Does The Test Work?

• The test is an ELISA .
• Enzyme Linked Immunosorbent Assay or EIA
  (Enzyme Immunoassay)

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ELISA
Standard Sandwich ELISA
S
Colour Change Substrate
Enzyme Conjugated Secondary Antibodies
Primary Detection Antibody
Antigen
Primary Capture Antibody
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The Lice Detection ELISA
S
Colour Change Substrate
Mouse Anti-Rabbit Enzyme Labelled Antibodies
Lice Protein 108 Captured From Comb and Cutter
Wash
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What are Magnetic Beads?

• Uniform, monosized (1µm) derivatised
  superparamagnetic polystyrene beads.
• Used as a solid support.
• Monoclonal antibody covalently linked to the
  surface.

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Advantages of Magnetic Beads

• Increased sensitivity due to
• Increased surface area of the beads means more
  Mab bound.
• Covalent bond of Mab to beads more robust than
  passive absorption.

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3. The magnetic beads travel through the sample
stripping out the lice protein.
Magnetic Bead ELISA
Plate ELISA
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4. Larger volumes of sample and washes
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5. Magnetic beads are drawn to the magnet for
washing and reagent changes
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The Lice Detection Laboratory Test
Washing Comb and Cutters
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The Test Kit
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The Comb and Cutter Sample before Clean-up
Lipid Layer
Coarse Sediment
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Sample After Filtration and Detergents
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Beads Sweep Through the Sample
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Beads are Drawn to the Magnet
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Samples Developed at 370 C
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Colour Development - Positive Samples
Beads
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Samples Submitted by Growers
Lice Mab
Negative Mab
Negative Field Samples
Negative Comb Cutter Control
Positive Comb Cutter Control