Use of the spectrophotometer

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Use of the spectrophotometer
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Picture of the spectrophotometer
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How to turn on the spectrophotomoter
Turn the button to 1 in the back of the machine
When warming up the spectrophotometer, there
should be no cuvettes in the machine
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Place about 500 mL of sample into the cuvette
use enough sample so the light can pass through
Wipe cuvette with a kimwipe to remove fingerprints
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Place cuvettes into the spectrophotometer

• There are 6 places put your blank in the space
  closest to you.
• Put the other samples in the next spaces.

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Go to first screen and select photometricand
then enter
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Then, enter your wavelengths

• Enter the number of wavelengths that you will use
  and the numbers of those wavelengths

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Press sample control (F4)

• Enter the number of samples you have
• Make sure the blank says yes
• Then return to this screen by pressing previous
  screen

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Go to the next screen by pressing F5
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The screen with the different wavelengths should
come up
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You should get readings close to zero for the
first sample which is the blank
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Press read to get absorbance values for your
sample
If the cuvettes do not shift automatically,
adjust the cell number to read the different
samples
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Record your values or press print to get a
printout

• If you have more than 5 samples, you will need to
  repeat the process
• Turn off the machine