Vitamin A

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(No Transcript)
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Vitamin A?
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Transgenic Rice
Output Trait Vitamin A
Cell
Input Trait
Make New Protein
New Trait!!
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Geranyl geranyl diphosphate colorless gene
enzyme phytoene
synthase Carotene phytoene
(______) gene Lycopene (_____) gene
Beta carotene (yellow)
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Transgene Design
Promoter? coding region?
Beta carotene (yellow)
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Geranyl geranyl diphosphate white gene psy
enzyme phytoene
synthase Carotene phytoene (pink) gene
crt1 enzyme phytoene
desaturase Lycopene
(red) geneIcy enzyme lycopene
B-cyclase
Beta carotene (yellow)
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Source of these genes gene psy
daffodil gene crt1 bacteria geneIc
y daffodil
Beta carotene (yellow)
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Transgene Design
Promoter? coding region?
Beta carotene (yellow)
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Transgene Design endosperm specific psy
coding region
Beta carotene (yellow)
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Transgene Design endosperm specific psy
coding region 35S crt1 coding
region
Beta carotene (yellow)
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Transgene Design endosperm specific psy
coding region 35S crt1 coding
region endosperm specific Icy coding region
Beta carotene (yellow)
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Geranyl geranyl diphosphate gene psy
enzyme phytoene
synthase Carotene phytoene (no
color) gene crt1 enzyme phytoene
desaturase Lycopene
(red) geneIcy enzyme lycopene
B-cyclase
Beta carotene (yellow)