What is Oxidative Stress

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What is Oxidative Stress?

• Reactive oxygen species
• Superoxide (O2)
• Hydroxyl radical (OH)
• Hydrogen peroxide (H2O2)

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Why study Oxidative Stress?

• Disorders
• ALS (Lou Gehrigs disease)
• Alzheimers
• Parkinsons
• Cancer
• AIDS
• Cardiovascular disease

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Yeast Strains

• Dr. Kim Slekar
• Wild Type
• Two Different Mutant Strains
• Pho85D
• ZMS1D
• Both involved in oxidative stress

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Pho85

• WT Resistant to oxidative stress
• Zwf1D Sensitive to oxidative stress
• Zwf1D/pho85D Resistant to oxidative stress
• Pho85D??CTT1 (catalase) ?suppress
  zwf1D
• ?HSP12 (heat shock protein)
• ?UBI4 (polyubiquitin)

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ZMS1

• Potential Transcription Factor?
• WT Resistant to Oxidative Stress
• ZWF1D Sensitive to Oxidative Stress
• ZWF1D/ZMS1 Resistant to Oxidative Stress

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Yeast Spheroplasts
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mRNA strand
3_____________________
To Dr. Malcolm Campbell
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Chip Reader
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.tiff data
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Scanalyze/gridding
 
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Troubleshooting