Classification of Microorganisms

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Chapter 10

• Classification of Microorganisms

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Taxonomy

• Three domains of living things
• Eukarya
• Bacteria
• Archaea

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Classification of organisms

• Nomenclature
• Binomial nomenclature
• Genus
• species

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Classification of organisms

• Kingdom
• Phylum or division
• Class
• Order
• Family
• Genus
• Species

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Classification of prokaryotes

• Bergys Manual of Determinative Bacteriology

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Classification of eukaryotes

• Protista
• Fungi
• Plantae
• Animalia

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Classification of viruses

• Not in a living kingdom

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Methods of classifying and identifying
microorganisms

• Morphological characteristics
• Differential staining

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Methods of classifying and identifying
microorganisms

• Morphological characteristics
• Differential staining
• Biochemical tests

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Methods of classifying and identifying
microorganisms

• Morphological characteristics
• Differential staining
• Biochemical tests
• Serology
• Serovars

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Methods of classifying and identifying
microorganisms

• Morphological characteristics
• Differential staining
• Biochemical tests
• Serology
• Phage typing

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Methods of classifying and identifying
microorganisms

• Morphological characteristics
• Differential staining
• Biochemical tests
• Serology
• Phage typing
• Fatty acid profile
• Flow cytometry

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Methods of classifying and identifying
microorganisms

• DNA base comparison
• DNA fingerprinting

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Methods of classifying and identifying
microorganisms

• DNA base comparison
• DNA fingerprinting
• Polymerase chain reaction (PCR)
• Nucleic acid hybridization

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Methods of classifying and identifying
microorganisms

• DNA base comparison
• DNA fingerprinting
• Polymerase chain reaction
• Nucleic acid hybridization
• Ribosomal RNA sequencing
• Fluorescent in-situ hybridization

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Using information from various tests

• Dichotomous keys
• Cladograms

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Using information from various tests

• Dichotomous keys
• Cladograms
• Numerical taxonomy