IVOS

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IVOS CEROSComputerAssistedSpermAnalysis
Spermatology 2006
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What is CASA?

• An automated system designed to
• Visualize and digitize successive images of sperm
• Process and analyze the images
• Provide precise and meaningful information on
  sperm kinematics and counts
• Combination of hardware and software
• Hardware optics, illuminator, digitizer,
  computer
• Software modules for motility, morphology, and
  viability analysis

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2 Current CASA Models from HT

• IVOS Sperm Analyzer
• Built-in optical system
• Automated, heated stage
• Fastest analysis 6 fields in 6 seconds
• CEROS Sperm Analyzer
• Complete system
• Includes negative phase contrast microscope

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NEW!! Modular CEROS

• Choose onlythe options you need
• Cost-effective
• Use your own negative or positivephase contrast
  microscope
• Same proven and trustedanalysis algorithms

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Multi-species Compatibility

• Human
• Non-human primates
• Domestic / companion animals
• Production animals
• Endangered species
• Laboratory rodents
• Sea mammals and fish

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Customized CEROS for Fish Sperm

• Cooled stage for optimum maintenance of fish
  sperm motility
• Applications include
• Monitoring sperm motility at hatcheries
• Testing extension media and spermiation stimulant
• Monitoring environmental pollutants
• Reproductive toxicology
• Fish, sea urchin, etc.

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Negative Phase Contrast - Standard
10x Negative Phase
20x Negative Phase
Provides fastest analysis
Provides superb visualization
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Getting Ready for Analysis (IVOS)

• Load sample in fixed or known depth chamber (e.g.
  Leja Chamber, Microcell, Makler)
• Place filled chamber on stage
• Select multiple analysis fields
• Press Start Scan

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Sperm Analysis

• Captures multiple snapshots extremely quickly
  (e.g., 30 images in 0.5 second).
• Tracks the motile cells from snapshot to snapshot
• Static sperm are identified based on motile cell
  means (size, contrast, intensity)
• Computer calculates results

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Results

• Motility
• Progressive Motility
• Concentrations
• Counts
• Velocity - 3 types
• Motion parameters

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Playback for Quality Control

• Motile sperm marked with green track
• Progressive sperm marked with blue track
• Slow sperm marked with purple track
• Static sperm marked with red dot

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Motile Playback Sample
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Research Options

• Obtain data on individual tracks (Edit Tracks)
• Study sub-populations of sperm (Sort)

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Data Management

• Clinical Filing
• Custom designed reports
• Store reports to disk
• HDATA ASCII Export
• Export data in database / spreadsheet compatible
  format
• Summary and individual track data

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Digital Image Storage

• Ability to save all fields analyzed
• Recall and reanalyze fields
• Sequential field analysis all fields from
  original analysis automatically analyzed in order
• Share images with other IVOS/CEROS users
• Create QC images for training purposes

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Morphology Options (Automated)

• Dimensions Morphology
• Human sperm only
• Analysis based on Dr. Krugers Strict Criteria
• Diff-Quik and PAP stains
• Metrix Morphology
• Multi-species
• Analysis based on users definition of normal
• Diff-Quik, PAP and other high contrast stains

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Real-Time Morphology (non-automated)

• Subjective (visual classification)
• Fast REAL-TIME
• Use non-stained, immobilized sperm, or standard
  stained smear
• Results combined with motility results
• Morph-merge option

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IDENT Fluorescence (IVOS Only)

• Static and motile sperm identification for
  high-detritus samples
• Sperm stained with Hoechst 33342 stain
• All sperm fluoresce under UV light
• Accurate counts to within 1

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VIADENT Fluorescence (IVOS Only)

• Sperm stained with Hoechst 33258 stain
• Only NON-VIABLE sperm fluoresce under UV
  illumination
• Counting
• Motile/Total counts under standard illumination
• Viable count under fluorescent illumination
• Only system to perform motility and viability
  analysis on the same, live sample

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Benefits of CASA

• Objectivity Removes technician bias
• Accuracy Best method for determining percent
  motility, percent progressive motility, and
  velocity parameters
• Precision Repeatable results
• Sensitivity Detects even the slightest changes
  in sperm motion
• Reliability Does not get tired eyes can
  analyze samples all day without sacrificing
  accuracy

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Hamilton Thorne Contact Information

• Phone [phone]
• Fax [phone]
• E-mail info_at_hamiltonthorne.com
• Web www.hamiltonthorne.com
• 100 Cummings Center, Suite 465EBeverly, MA 01915
  USA