Prostate: Transverse section

1
Prostate Transverse section
2
Radical Prostatectomy
Bladder neck
Apex
3
Radical Prostatectomy
Base/ Bladder Neck
Apex
Posterior quadrants of alternate slices have been
removed and frozen after inking the surface
4
Lateral aspect
Schematic of how samples of tumor are obtained
from a radical prostatectomy specimen, without
compromising sampling or diagnostic pathology data
Seminal Vesicle
Permanent section
Permanent section
right
left
1
7
2
6
3
5
4
5R
5
5R 5L
5L
Fresh, non-frozen tissue for flow sorting
Frozen section
Unfixed tissue for RNA DNA
left
right
4R
Frozen section
4
4R 4L
4L
Permanent section
left
right
permanent section
3R
3
3R 5L
3L
Prostate carcinoma
5
Human prostate (lateral view)
Obtaining samples of non-tumor tissue from a
radical prostatectomy specimen.
Seminal Vesicle
Permanent section
Fixed tissue In-situ hybridization
right
1
left
7
2
6
3
5
4
5R
5
5R 5L
5L
Fresh, non-frozen tissue cell sorting
This block is snap frozen (isopentane in liquid
N2), within lt 20 minutes of devitalization
Frozen tissue Immunostains Laser capture
right
left
4R
4
4R 4L
4L
Frozen section
Permanent section
left
right
Permanent section
3R
3
3R 5L
3L
Prostate carcinoma
6
Prostate (transverse section)
7
Normal prostate parenchyma (high magnification)
Basal epithelial cells
Secretory (luminal) epithelial cells
Fibromuscular stromal cells
8
Normal prostate parenchyma (low magnification)
fibromuscular stroma
lymphocytes
normal glands