Metabolic Biochemistry BIBC102

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Metabolic Biochemistry BIBC102 Lecture
13 November 2, 2007
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For the MIDTERM on Tuesday the following rooms
are assigned A G CENTR 115 H Z CENTR
212
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the only membrane- bound enzyme in the TCA cycle
LNC Fig.16.7
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the Krebs cycle is amphibolic
LNC Fig.16.15
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regulatory molecules NADH ATP, ADP Ca2 key
allosteric enzymes the dehydrogenases citrate
synthase
LNC Fig.16.18
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THE GLYOXYLATE CYCLE
LNC Chapter 16, pages 623-626
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the only membrane- bound enzyme in the TCA cycle
LNC Fig.16.7
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C2
C4
C6
C4
C6
C4
C2
C2
LNC Fig.16-20
Note that the figure 16-16 in the 3rd edition has
an error (there is an O instead of an H)
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cellulose
LNC Fig.16.22
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isocitrate lyase inactivated by low ATP (high
AMP/ADP)
isocitrate dehydrogenase inactivated by
phosphorylation
LNC Fig.16.23
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ELECTRON TRANSPORT AND OXIDATIVE PHOSPHORYLATION
Chapter 19 in LNC
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CO2
Guinea Pig
Charcoal
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..this fire stolen from heaven, this torch of
Prometheus, does not only represent an ingenious
and poetic idea, it is a faithful picture of the
operations of nature, at least for animals that
breathe... the torch of life lights itself at the
moment the infant breathes for the first time,
and it does not extinguish itself except at
death. Lavoisier, 1789
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Carbohydrate oxygen gt carbon dioxide and
water
C6H12O6 6 O2 gt 12 H2O 6 CO2
ENERGY
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Overview of glycolysis and the Krebs cycle
A) Glucose 2ADP 2 Pi 2
NAD gt 2 Pyruvate 2 ATP 2 NADH B)
2 Pyruvate 2 NAD 2
CoA gt 2 acetyl-CoA 2 NADH 2 CO2 C)
2 acetyl-CoA 2x3 NAD 2 FAD
gt 4 CO2 6 NADH 2 FADH2 D)
2 GDP
2 Pi gt 2 GTP
There is no consumption of oxygen so far
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2 NADH 2 H O2 gt 2 NAD 2 H2O
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RESPIRATION
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2
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Wild type SDHC
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Outer membrane
Inner membrane
Matrix
Intermembrane space
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LNC Fig.19.7
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the arrangement of the complexes in the inner
membrane and the order of electron flow
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Red the components are drained of electrons,
i.e. oxidized
Blue the components are filled with electrons,
i.e. reduced
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succinate
Succinate dehydrogenase membrane bound enzyme
of Krebs cycle
Four integral membrane protein complexes Two
mobile carriers ubiquinone and cytochrome c
LNC Fig.19.15
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LNC Fig.19.8
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oxidized Coenzyme Q or Q
reduced Coenzyme Q or QH2
LNC Fig.19.2
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Heme apoprotein cytochrome
LNC Fig.19.3
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Structure of cytochrome c
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LNC Fig.19.4
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NON-HEME IRON - SULFUR CENTERS Fe2-S2 Fe4 -
S4
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LNC Fig.19.5
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Fe2-S2
LNC Fig.19.5
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Fe4-S4
LNC Fig.19.5
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LNC Fig.19.5
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O2
NADH
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End of Lecture 13 February 7, 2007