Stephen E' Fish, Ph'D'

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Stephen E. Fish, Ph.D. Mitchell L. Berk,
Ph.D. Marshall University J. C. E. School of
Medicine
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Note to instructors I use these PowerPoint
slides in histology lectures that I give to first
year medical students. Copy the slides, or just
the images into your own teaching media. We all
know that teaching science often requires
compromises and simplification for specific
student populations, or the requirements of a
specific course. Please feel free to offer
suggestions for improvements, corrections, or
additional illustrations. I would be pleased to
hear from anyone who finds my work useful, and am
always willing to make it better. Also, the
images have been compressed to screen resolution
to keep PowerPoint file size down, and I can
provide them at any resolution. Contact me about
the illustrations and Mitchell L. Berk about the
photomicrographs. Stephen E. Fish,
Ph.D. Fish_at_Marshall.edu Berk_at_Marshall.edu
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Adipose Tissue, Cartilage Bone
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In regions of fat, adipocytes look like chicken
wire
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Fat cells use sER to store release fat
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Comparebrown white
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Hyaline cartilage
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Hyaline cartilage
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Cartilage matrix consists of proteoglycan GAGs,
hyaluronic acid, type 2 collagen
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Collagen GAGs in cartilage are much more
concentrated than in loose CT
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Articular cartilage

• Joint cartilage, no perichondrium because its
  mostly a contact surface
• Bathed maintained by synovial fluid
• Collagen perpendicular to the surface (resists
  compression)
• But bends parallel (makes a wearing surface)

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Elastic cartilage

• Some of the type 2 cartilage replaced by elastic
  fibers
• Makes the cartilage much more flexible bends
  without breaking
• Found in outer ears, epiglottis, nose
• Takes an elastic stain to see (epiglottis in
  slide sets)

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Bone

• In its structure, your skeleton is comparable to
  light weight high tech composites used in
  aerospace applications with the tough fiber
  component made of collagen, the rigid binder
  made of calcium apatite crystals

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Bone
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Ground bone
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Ground bone
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Structure seen in ground bone
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More terminology
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Getting down to the microanatomy
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Ground bone
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Decalcified bone
Medullary cavity
Haversian canal
Osteocyte
Inner circumferential lamellae
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Periosteum
Cellular or osteogenic
Fibrous
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Bony trabecula with endosteum
Endosteum with Osteoblasts Stem cells Osteoclasts
Osteocyte
Osteoclast
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Osteoclasts osteoblast continually remodel bone
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Osteoclasts
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Remodeling New haversian systems
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Start a new osteon at the X(click through
sequence to see osteon form)
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The new channel for the osteon is being enlarged
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This is the widest point
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The osteoblasts are filling in the channel with
haversian lamellae
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At the trailing edge of the process, most of the
lamellae are in place
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As more new osteons are made older ones become
interstitial lamellae
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Side by side comparison
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Sherman says
I find the flavor of beef bone to be robust
assertive without being aggressive or
overbearing