Gel electrophoresis

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Gel electrophoresis

• L Mathias

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Definition

• Separation of DNA fragments according to size,
  based on movement through a gel medium when an
  electric field is applied.

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DNA negatively charged
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DNA cut up using restriction endonucleases
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Make up the gel which the DNA will be put into
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Dye added to the DNA
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Buffer solution added to the tank
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DNA samples loaded into wells
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Electrical current applied to the chamber
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DNA is stained using ethidium bromide
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