Glucose sensor

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Glucose sensor
lt Integrated Biotechnology Project ? gt

• ???????
• 20051294 ???

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Contents

• 1) Biosensor
• 2) Enzyne-sensor
• 3) Glucose sensor
• 4) ????
• 5) Cyclic voltammetry
• 6) Chronoamperometry
• 7) Discussion
• 8) Glucose sensor? ???

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Biosensor

• ?? ????? ??? ?????? ???? ???? ???? ??
• ?? ??? ???????? ?? ??? ??? ?? ???? ??? ??? ?? ?
  ??

?? ??? ?? ??? ??? ??, ?? ? ??? ???? ?? ???
??
????!!
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The Principle of Biosensor

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Enzyme-sensor

• 1962? Clark? ???? ????? ???
• ????? ??
• ?? ? ??? ??? ?????? ????
• ??? ?? ???? ???
• ??????? ???, ???

?? ??? ??? ??
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Glucose sensor

• Glucose oxidase? ???? ????? ??

GOD GOD GOD GOD GOD GOD
GOD GOD GOD GOD GOD GOD
Nafion

• ??? ???? ????, ?? ??? ???? ?? ?????? ???? ??? ?
  ??.
•  
• ????? ???? ??? ? ??.

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????

• 1) Cyclic voltammetry? chronoamperometry? ?? ???
  ????.
• 2) ?? ??? ???? ???? ??? ??? ??, ????
  ?????(biosensor)? ? ??(glucose sensor)? glucose
  oxidase(GOD)? ???? ????.
• 3) ??? ??? glucose sensor? ???? ??????? glucose?
  ????.

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Cyclic voltammetry

• ?? ??? ??? ??? ??? ?? ?? ????(potential sweep)?
  ??.
• ????? ??? ?? ??? ?? ?? ???? ? ?? ?? ???? ???? ???
  ???? ? ? ??.
• ??? ????? ? ???? ??? ??? ??? ??? ???? ??? ???? ??
  ?????? ??(Cyclic Voltammetry) ??.

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Cyclic voltammetry
(a)
(b) ?? ???? ???
?? ??(a)? ??-?? ??? ??(b)
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Cyclic voltammetry

• Fe?(CN)63- e- ? Fe?(CN)64-

n 0.843, 0.831, 0.776, 0.787, 0.678
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Cyclic voltammetry
Randles-Sevcik equation
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Chronoamperometry

• ??? ??? ?? ? ? ??? ?? ??? ??? ???? ??
• ????? ??? ??? ?? ???? ?? ? ? ??.

????(limiting current) Il nFAm0C0
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Chronoamperometry

• GODox glucose ? GODred gluconolacton
• GOD red O2 ? GOD ox H2O2
• H2O2 ? O2 2H 2e-

Glucose Oxdase
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Chronoamperometry
glucose ??(1mM)? ?? Current? ??? ?? ??
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Chronoamperometry
glucose ??? ?? Current? ??
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Chronoamperometry

• Michaelis - Menten equation
• Lineweaver-Burk equation

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Chronoamperometry
Lineweaver-Burk plot
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Other Methods of glucose sensor

• NSMM? ??? ???
• ??? ??? ?? ?? ??? ????? ??? ??? ???? ?????? ???
  ??? ??? ? ?? ????? ??
• ??? ??? ???? ?? ? ?????? ?? ???? ??? ???? ???
  ??
• ???? ??? ??? lab?? ?? ?????? ??? ?????? ?
  ??? ?? ??

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Reference

• 1) Mary K. Campbell, BIOCHEMISTRY 4th, THOMSON,
  2003, 117121
• 2) ????, ??? ????, ???, 1996
• 3) ???, ?????, ????, 1994
• 4) ??????, ????, ???, 2001
• 5)http//news.naver.com/news/read.php?modeLSDoff
  ice_id093article_id0000000745section_id105me
  nu_id105

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