HSQC???????

1
HSQC???????
?????????? ???
PEP-HSQC PEP?preservation of equivalent
pathways???,????HSQC?S?????t1????????????????????
??,???????INEPT????S90?????????????1H,????????,???
??????????????????,???????????PEP?????????????????
?????????,?????????
2
PEP-HSQC
?????????? ???
3
PEP-HSQC
?????????? ???
4
PEP-HSQC
?????????? ???
5
PEP-HSQC
?????????? ???
6
PEP-HSQC
?????????? ???
7
PEP-HSQC
?????????? ???
8
PEP-HSQC
?????????? ???
3. PFG-PEP-HSQC ????gradient-enhanced
HSQC,??????????????????,????????????,????????????
????????????????????PEP-HSQC????,??????????,??????
??????????
9
PEP-HSQC
?????????? ???
10
PEP-HSQC
?????????? ???
11
PEP-HSQC
?????????? ???
12
PEP-HSQC
?????????? ???
13
PEP-HSQC
?????????? ???
14
PEP-HSQC
?????????? ???
15
??NMR????? TROSY (Transverse relaxation-optimize
d spectroscopy)
?????????? ???

• ??-???????????????????????J???????????????????????
  ?????
• 1997?Pervushin?????????????,????????????,?????????
  ???????????????????????????????

16
TROSY
?????????? ???
17
TROSY
?????????? ???
18
???TROSY??
?????????? ???
19
TROSY
?????????? ???
20
TROSY
?????????? ???
21
TROSY
?????????? ???
22
TROSY
?????????? ???
23
???TROSY??-ST2-PT
?????????? ???
??????????FID,????? ?1y,-x?2-y?4-yfref1,2 ?
??FID?????1y,x?2y?4y???g2??
24
TROSY
?????????? ???
?. ?????? ?????p??S?TROSY??????????p?????-???????
??,??S????????????,??????????????????,???????????-
??????,????????,?????????????-????????????TROSY???
???????????????
25
TROSY
?????????? ???
???15N-1H??,??H-16 ppm,??N-160 ppm,rHN0.101
nm,?CSA??????rHN??????,????????????1H?????1100
MHz?,p??S??p??I????????,??????????????????,???????
????1H????CH???????-??????,?????CH??1H??2H,TROSY??
???????
26
TROSY??????????
?????????? ???

• ????????1H?????1.1GHz?,15N-1H???TROSY?????
• ???CH??1H??2H,TROSY???????

750 MHz????????????
?????? 1H??(?) 1H??(?) 15N??(?) 15N??(?)
150 kDa 15 Hz 70 Hz 5 Hz 60 Hz
800 kDa 50 Hz 350 Hz 15 Hz 300 Hz
27
TROSY???
?????????? ???
HSQC
28
TROSY
?????????? ???
??????TROSY?????,????TROSY???????,???????????????,
???????????? ???????15N-1H???,????13C????????????
?,?????TROSY???13C-1H???
29
Problem set 1
?????????? ???

• ???IPAP-HSQC?????,???????(??????,?????,????,??,???
  ?,FID???)
• ?????,????N-H?????????????????
• ??email?? kruan_at_ustc.edu.cn, due on 2pm, May 7,
  2014.