3. ?mRNA???????????????????? - PowerPoint PPT Presentation

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3. ?mRNA????????????????????

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Title: 1 Last modified by: 530La Created Date: 11/26/2001 1:28:29 PM Document presentation format: Company: fxn Other titles – PowerPoint PPT presentation

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Title: 3. ?mRNA????????????????????


1
???????????????
2
??????????? ???????????? ?????????????? ??????????
?? ?????????????
3
?????? 1. 20?????,????????????????? 2.
????????????,?????????????? 3. ?mRNA??????????????
?????? 4. ?????????????,??????????????????????,??
?????????????????????????DNA??????
4
5. ??????????????????? 20??90????,?????????
??? 6. ?????????? (1)????1994???,???????1995?7??
Electrophoresis???????????????????,?????????????
???????????? (2)????????????????????????????
5
7. ???????????????? (1)???????????????????
???????? (2)????,???????????,??
??????????? (3)?????????,?????????
???????????????
6
  • ????????????
  • ?????????????????
  • (???????) ????2-D??
  • 2. ???????????(???????)
  • X-????????(??????)??????????????????
    ?????(?????)?
  • 3. ???????????(?????????)
  • ???????????????,????????????????????
    ???
  • 4. ????????(???????)
  • ???????????????????

7
????????????? (?)??????????????????
2-D?? 1. 1975?,Patrick
OFarrel?????????? 2. ?? (1)????
(2)???? (3)???SDS-PAGE
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3. 2-D??????? (1)??Western blot?? (2)Edman?????
(3)?????(peptide-mass fingerprinting)??
?? (4)???????
17
2D ?? ?  Mass ????
18
(?)?????? 1. ??????????????X-????????(??????)????
???????????????????(?????)? X-????????????????
??? (1)??? (2)???????????????????????????????????
???????????? 2. ???????????????????????? (1)?????
?????????,?????????,????????????????? (2)?????????
????????,??????????????,?????,??????????????
19
(?)??????? 1. ?????????? 2. ??????????
(1)????????????????? (2)????????
(3)????????,??????,????? (4)Edman??
20
????????? 1.
?????Sanger??(20??50??)? 2. ????????????????(
20??50??)? 3. Edman??(20??70????)?
????????????????????????
??20??80??,?????????????????????????,?????10pmol??
,??????????????80?????????????????????? 4.
20??70??,????????????Edman??DABITC,?????????????
?
21
5. ?2-D???????????????PVDF(??????)??,????????????
?????Edman??? 6. ???????(20??90?????????????)???,?
???N?????????????????????????HPLC?????????10fmol??
????????????????????????????????????????
22
7. ???C??? C????????PCR????????????????C????????
??????????????????????? (1)????????Schlack???????
???? ????????? (2)??20??90??????????????
?? ???,???HPLC?????????(TH)
???,??????????????????
?C????,??????6-8????C??? ???
23
8. ???????????? (1)???DNA???????????????????????
????,Edman??????N?????????,??????????????? (2)20??
80????????????(FAB??)??????????????????????????FAB
??????????????????,???????????????????????,???????
?????
24
(3)20??80??????????????????????-??????? ??????????
???????????????,???????HPLC???????,???????????????
??HPLC????????????????? ????????????30????????
????????????????????,?????????????????????????????
????????????????????C??N??????,?????????????????(1
-2pmol),?????,????????????
25
9. ??????????Edman?????????????
???????????? 10. ??????? (1)N ?????????????? (2)C
????????????????????????? (3)???????????????? (4)?
?????????????????????,?? ????????
26
(?)??????????????? 1. ???????????????????????????,
????2-D?????????????????????????,??????????????? 2
. ??????????????,??????????????????? (?)?????????
??? ????????????????????????????????????
27
???????????? 1. ???????????????? 2.
??????????,????????????? ?????????????? 3.
?????????????????? 4. ?????????????????,??????
???????,?????????????? 5. ???????????,???????????
? ???????
28
????????????? 1. 2-D??????????????????
?,????????? 2. 2-D????????????? 3.
??????????18???????? 2-D????? 4.
????????????,????2-D? ?????????????????
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