BC basic - from big bang

1
Central Dogma
DNA
? ?
Replication
Reverse Transcription
? ?
???
Transcription
RNA
? ?
Translation
Protein
Juang RH (2004) BCbasics
2
Template (-) Strand Transcribes to mRNA
nontemplate strand
() strand
coding strand
5
3
ATG
TAG
Codon is denoted on mRNA
AUG
UAG
RNA
5
3
TAC
ATC
5
3
template strand
(-) strand
Juang RH (2004) BCbasics
3
Both DNA Strands Could Be Expressed
RNA is synthesized in 5? 3 direction
Adenovirus fully utilizes its genomic sequence
Juang RH (2004) BCbasics
4
Antisense RNA Inhibits the Expression
Nontemplate


Antisense Gene
-
Antisense RNA
X
RNA/RNA Hybrid

mRNA
-
Template
Juang RH (2004) BCbasics
5
Create an Antisense RNA
Gene Addition
Transcription
Normal mRNA
Antisense RNA
RNA double helix
mRNA translation inhibited
Adapted from Alberts et al (2002) Molecular
Biology of the Cell (4e) p.537