1. ???(arecoline) ,????(arecaidine)

1
??????????????

• ? ? ?????????????????
• ??? ??
• ??????????????????
• ??? ??

2
??????????
3
????

• 1. ????---?????????
• 2. ????---??????????

4
?????????

• 1. ????
• 2. ????
• 3. ????
• 4. ????
• 5. ???

5
????????????

1. ??????
2. ???

6
?????????

• 1. ???(arecoline) ,????(arecaidine)
• 2. ?????????,????(nitrosamine)
• 3. ???????????????????????????????
• 4. ???????(safrole)

7
???

• 1. ?????
• 2. ???

8
?????

1. ???????????
2. ?????????
3. ?????88???????
4. ???????? 2200?
5. ???????? 4000?
6. ????????????????
7. ????????????????

9
??????

• 1. ???
• 2. ????
• 3. ???
• 4. ????
• 5. ???????

10
????

• 1. ???
• 2. ???
• 3. ????????
• 4. ????
• 5. ???

Homogeneous leukoplakia ????
Non-homogeneous Leukoplakia ?????
11
?????

• 1. ??? --- ?0?
• 2. ??? --- 1-7?
• 3. ???????? --- 4 - 15?
• 4. ??? --- 28?(18 - 47?)
• 5. ?????? --- 4 - 11?
• 6. ?????? --- 20 - 35?

12
????????

• 1. ????????? --- 90?
• 2. ???????? --- 5?
• 3. ???? --- 5?

13
????????

• 1. ????????? --- 10?
• 2. ?????????? --- 40?
• 3. ???? --- 50?

14
?????????

• 1. ??????
• 2. ????
• 3. ??????
• 4. ?????

15
???????

• 200???

16
?????????

• 1. ??????3?
• 2. ?????6-8??

17
???????????

• 1. 10??? --- 2.3?????
• 2. 15??? --- 4.5?????
• 3. 17??? --- 7.6?????

18
?????????????

• ?????
• ??????
• ????
• 3. ??????
• 4. ??????

19
1????????

1. ? DNA ????????
2. ???????????

20
1 Toluidine blue solution

1. Toluidine blue O powder -- 1 g
2. Acetic acid ------------------- 10 ml
3. Absolute alcohol ----------- 4.2 ml
4. Distilled water -------------- 86 ml

21
1 Toluidine blue vital stain

• Procedures for toluidine blue vital stain
• 20 ml 1 acetic acid -- oral rinse for 20 sec
• 10 ml 1 toluidine blue solution oral rinse for
  30 sec
• 20 ml 1 acetic acid -- oral rinse for 20 sec
• Clean water oral rinse several times
• Evaluation of the lesion deep-blue stain
  represents positive result.

22
1 Toluidine blue vital stain
False positive reactions can occur due to dye
retention by filiform papillae and oral ulcers,
as well as mechanically in fissures.
23
????????
24
? 330 nm ????

• ????????????
• ????????????
• ???380 nm ?
• ????460 nm?

25
????????????

• 1. 380-nm?? ??????????????????
• 2. 460-nm?? ???????????NADH???

26
Groups Case. No. A46010 nm/A38010 nm ????? Unpaired Students t-test between groups P value
??? ??? 15 0.76 0.07 Between NOM and OSF samples Plt0.001
???????? 30 0.56 0.05 Between NOM and EH samples Plt0.001
?????? 25 0.97 0.17 Between NOM and ED samples Plt0.001
?????? 15 1.01 0.19 Between NOM and SCC samples Plt0.005
??????? 13 1.54 0.75 Between SCC and EH samples Plt0.001
Between SCC and ED samples Plt0.001
Between SCC and OSF samples Plt0.001
Between EH and ED samples Pgt0.05
27

• Thank you
• for
• your attention.