Flow

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Flow Cytometry
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Flow Cytometry
Laser
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Forward Scatter
Forward Scatter indicates size
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Side Scatter
Side Scatter indicates complexity
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Fluorescence Measurements
FITC labeled cell!
Fluorescent wavelength detector
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Fluorescence Measurements
PE labeled cell!
Fluorescent wavelength detector
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Fluorescence Detection

• FL-1 FITC, CFSE, ALEXA
• FL-2 PE, PI
• FL-3 Texas Red, Red-670
• FL-4 APC, Topro

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Single Staining
This sample has a single stain of CD4 labelled
with FITC stain, giving a green color to those
cells expressing CD4.
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Single Staining
CD8PE
No Stain
This sample has a single stain of CD8 labelled
with PE stain, giving an orange color to those
cells expressing CD8.
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Double Staining
CD8PE
CD4FITC
This sample has a double stain of CD4FITC and
CD8PE, giving a green and orange color to those
cells expressing both CD4 and CD8.